Overview

  • Product name
    Anti-TOMM22 antibody
    See all TOMM22 primary antibodies
  • Description
    Mouse monoclonal to TOMM22
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-143 of Human TOMM22

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab179826 as a replacement.

Properties

Applications

Our Abpromise guarantee covers the use of ab57523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 15 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Cellular localization
    Mitochondrion outer membrane; Single-pass membrane protein
  • Database links
    • Alternative names
      • 1C9 2 antibody
      • hTom 22 antibody
      • hTom22 antibody
      • Mitochondrial import receptor subunit TOM 22 homolog antibody
      • Mitochondrial import receptor subunit TOM22 homolog antibody
      • Mitochondrial import receptor Tom 22 antibody
      • Mitochondrial import receptor Tom22 antibody
      • MST 065 antibody
      • MST065 antibody
      • MSTP 065 antibody
      • MSTP065 antibody
      • TOM 22 antibody
      • TOM22 antibody
      • TOMM 22 antibody
      • Translocase of outer membrane 22 kDa subunit homolog antibody
      • Translocase of outer mitochondrial membrane 22 homolog antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22 antibody (ab57523)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22 antibody (ab57523)
      TOMM22 antibody (ab57523) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human small Intestine.
    • Western blot - Anti-TOMM22 antibody (ab57523)
      Western blot - Anti-TOMM22 antibody (ab57523)
      TOMM22 antibody (ab57523) at 1ug/lane + NIH/3T3 cell lysate at 25ug/lane.
    • Immunocytochemistry/ Immunofluorescence - Anti-TOMM22 antibody (ab57523)
      Immunocytochemistry/ Immunofluorescence - Anti-TOMM22 antibody (ab57523)
      ICC/IF image of ab57523 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57523, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Flow Cytometry - Anti-TOMM22 antibody (ab57523)
      Flow Cytometry - Anti-TOMM22 antibody (ab57523)
      Overlay histogram showing HeLa cells stained with ab57523 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57523, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    References

    This product has been referenced in:
    • Zeitlow K  et al. The biological foundation of the genetic association of TOMM40 with late-onset Alzheimer's disease. Biochim Biophys Acta 1863:2973-2986 (2017). Read more (PubMed: 28768149) »
    • Shi R  et al. BNIP3 Interacting with LC3 Triggers Excessive Mitophagy in Delayed Neuronal Death in Stroke. CNS Neurosci Ther N/A:N/A (2014). Mouse . Read more (PubMed: 25230377) »

    See all 8 Publications for this product

    Publishing research using ab57523? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    4 reviews or Q&A

    10
    Votes: Highest First

    not getting specific band for TOMMs

    Hello!

    We also do recommend using RIPA buffer rather than NP40 for mitochondrial targets, would this be something you are able to do with your samples?

    - ab57523 giving 2 prominent bands at 30kDa and 88 kDa.
    - Used TOMM22 as marker for outer mitochondrial membrane proteins
    - What is the identity of 88 kDa band?
    - protease inhibitors used
    ab48139
    - mouse cells used for mitochondrial...

    Read More

    Thank you very much for your patience while I was in discussion with lab.

    My colleague has confirmed that, they tested ab57523 with PC-12, Raw 264.7 and NIH/3T3 cells in western blot and they haven't observed any band at 88kDa. The band they o...

    Read More

    It appears the protein name in Yeasts is TOM22 and not TOMM22. Below is it's DNA sequence. The currently selected gene/sequence is : TOM22/YNL131W on chromosome XIV from coordinates 378767 to 379225. This is the genomic DNA sequenc...

    Read More

    Thank you for providing this extra information. The alignment shows a very low homology between the yeast protein and the human TOMM2 protein (www.uniprot.org/uniprot/Q9NS69), 16% only. This result suggests that unfortunately the TOMM2 ...

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    Dear Tech support,   What are the chances of antibodies ab57523 and ab15575 (or another sec61 antibody) of working with Yeast's proteins WB based on immunogen sequence homology? Thank you for your kind help. Regards,

    Thank you very much for your inquiry. Unfortunately we do not have an antibody in our catalogue for the moment for which we can suggest a crossreactivity with the yeast SEC61 proteins. I am very sorry for this. Please see further below for the alignmen...

    Read More

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