- Datasheet
- Specific References (6)
- Protocols
Overview
- Product nameAnti-TOMM22 antibody
See all TOMM22 primary antibodies - DescriptionMouse monoclonal to TOMM22
- Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
- Species reactivityReacts with: Mouse, Human
- Immunogen
Recombinant full length protein, corresponding to amino acids 1-143 of Human TOMM22
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
PBS, pH 7.2 -
Concentration information loading... - PurityProtein G purified
- ClonalityMonoclonal
- IsotypeIgG2a
- Light chain typekappa
- Research areas
Associated products
- Compatible Secondaries
- Isotype control
Applications
Our Abpromise guarantee covers the use of ab57523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 15 kDa. | |
| IHC-P | Use a concentration of 3 µg/ml. | |
| Flow Cyt | Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. | |
| ICC/IF | Use a concentration of 10 µg/ml. |
Target
- Cellular localizationMitochondrion outer membrane; Single-pass membrane protein
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Database links
- Entrez Gene: 56993 Human
- Entrez Gene: 223696 Mouse
- Omim: 607046 Human
- SwissProt: Q9CPQ3 Mouse
- Unigene: 285005 Human
- Unigene: 246435 Mouse
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Alternative names
- 1C9 2 antibody
- hTom 22 antibody
- hTom22 antibody
see all
Anti-TOMM22 antibody images
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IHC-P - TOMM22 antibody (ab57523)TOMM22 antibody (ab57523) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human small Intestine. -
Western blot - TOMM22 antibody (ab57523)
Predicted band size : 15 kDa
TOMM22 antibody (ab57523) at 1ug/lane + NIH/3T3 cell lysate at 25ug/lane. -
Immunocytochemistry/ Immunofluorescence-TOMM22 antibody(ab57523)ICC/IF image of ab57523 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57523, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Flow Cytometry-TOMM22 antibody(ab57523)Overlay histogram showing HeLa cells stained with ab57523 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57523, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
References for Anti-TOMM22 antibody (ab57523)
This product has been referenced in:
- Shi R et al. BNIP3 Interacting with LC3 Triggers Excessive Mitophagy in Delayed Neuronal Death in Stroke. CNS Neurosci Ther N/A:N/A (2014). Mouse . Read more (PubMed: 25230377) »
- Brinkmann CR et al. Mitochondria and the lectin pathway of complement. J Biol Chem 288:8016-27 (2013). Flow Cyt ; Rat . Read more (PubMed: 23378531) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"