Overview

  • Product nameAnti-TOMM34 antibody
    See all TOMM34 primary antibodies
  • Description
    Rabbit polyclonal to TOMM34
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-309 of Human TOMM34 (NP_006800.2).

  • Positive control
    • Mouse testis tissue lysate. Hela NE.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab103585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 35 kDa.

Target

  • FunctionPlays a role in the import of cytosolically synthesized preproteins into mitochondria. Binds the mature portion of precursor proteins. Interacts with cellular components, and possesses weak ATPase activity. May be a chaperone-like protein that helps to keep newly synthesized precursors in an unfolded import compatible state.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the Tom34 family.
    Contains 6 TPR repeats.
  • Cellular localizationCytoplasm. Mitochondrion outer membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • hTom34 antibody
    • HTOM34P antibody
    • Mitochondrial import receptor subunit TOM34 antibody
    • OTTHUMP00000031079 antibody
    • Outer mitochondrial membrane translocase (34kD) antibody
    • TOM 34 antibody
    • TOM34 antibody
    • TOM34_HUMAN antibody
    • TOMM 34 antibody
    • TOMM34 antibody
    • Translocase of outer membrane 34 kDa subunit antibody
    • Translocase of outer mitochondrial membrane 34 antibody
    • URCC 3 antibody
    • URCC3 antibody
    see all

Anti-TOMM34 antibody images

  • Anti-TOMM34 antibody (ab103585) at 1/500 dilution + Mouse testis tissue lysate at 50 µg

    Predicted band size : 35 kDa
  • All lanes : Anti-TOMM34 antibody (ab103585) at 1/500 dilution

    Lane 1 : TOMM34 transfected 293T cell lysate
    Lane 2 : 293T non-transfected cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 35 kDa
  • Anti-TOMM34 antibody (ab103585) at 1/500 dilution + HeLa NE at 50 µg

    Predicted band size : 35 kDa

References for Anti-TOMM34 antibody (ab103585)

ab103585 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Heart)
Permeabilization Yes - Tween-20
Specification Heart
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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Submitted Aug 29 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Heart)
Gel Running Conditions Reduced Denaturing (4-12%)
Loading amount 20 µg
Specification Heart
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jul 29 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"