Overview

  • Product nameAnti-TOMM40 antibody
    See all TOMM40 primary antibodies
  • Description
    Rabbit polyclonal to TOMM40
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide surrounding amino acid 339 of human TOMM40.

Properties

Applications

Our Abpromise guarantee covers the use of ab51884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 38 kDa.

Target

  • FunctionChannel-forming protein essential for import of protein precursors into mitochondria.
  • Sequence similaritiesBelongs to the Tom40 family.
  • Cellular localizationMitochondrion outer membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C19orf1 antibody
    • D19S1177E antibody
    • Haymaker protein antibody
    • Mitochondrial import receptor subunit TOM40 homolog antibody
    • Mitochondrial outer membrane protein antibody
    • Mitochondrial outer membrane protein TOM40 antibody
    • p38.5 antibody
    • PER EC1 antibody
    • PEREC1 antibody
    • Probable mitochondrial import receptor subunit TOM40 homolog antibody
    • Protein Haymaker antibody
    • TOM40 antibody
    • TOM40_HUMAN antibody
    • TOMM40 antibody
    • Translocase of outer membrane 40 kDa subunit homolog antibody
    • Translocase of outer mitochondrial membrane 40 antibody
    • Translocase of outer mitochondrial membrane 40 homolog (yeast) antibody
    • Translocase of outer mitochondrial membrane 40 homolog antibody
    • Translocase of outer mitochondrial membrane 40, yeast, homolog of antibody
    see all

References for Anti-TOMM40 antibody (ab51884)

This product has been referenced in:
  • Nautiyal M  et al. Differences in oxidative stress status and expression of MKP-1 in dorsal medulla of transgenic rats with altered brain renin-angiotensin system. Am J Physiol Regul Integr Comp Physiol 303:R799-806 (2012). Read more (PubMed: 22914751) »
  • Goemans CG  et al. Intra-mitochondrial degradation of Tim23 curtails the survival of cells rescued from apoptosis by caspase inhibitors. Cell Death Differ 15:545-54 (2008). Read more (PubMed: 18174902) »

See all 2 Publications for this product

Product Wall

This antibody is successfully tested with mouse samples in western blot hence guaranteed for all mouse samples provided the protein is expressed. We do not provide small sample for testing however if this antibody doesn't show expected results a free r...

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Hello!

We also do recommend using RIPA buffer rather than NP40 for mitochondrial targets, would this be something you are able to do with your samples?

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (NIH/3T3 fibroblasts)
Loading amount 20 µg
Specification NIH/3T3 fibroblasts
Gel Running Conditions Reduced Denaturing (Nupage 10% Bis-Tris Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Nov 25 2009

Thank you for your enquiry. This TOMM40 antibody mainly detects a band at around the 40 kDa band and a smaller isoform (~30 kDa). It may also detect large molecular weight bands at lesser extent on reducing gel. I hope this information will be h...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"