Overview

  • Product nameAnti-Tomosyn antibody
    See all Tomosyn primary antibodies
  • Description
    Rabbit polyclonal to Tomosyn
  • Tested applicationsSuitable for: WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Cow, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 850 - 950 of Human Tomosyn.

    (Peptide available as ab45226.)

  • Positive control
    • WB: brain tissue lysate IHC: brain (hippocampus)

Properties

Applications

Our Abpromise guarantee covers the use of ab41271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 122,128 kDa (predicted molecular weight: 128 kDa).
IHC-FoFr 1/1000 - 1/3000.

Target

  • RelevanceTomosyn plays a regulatory role in calcium-dependent exocytosis and neurotransmitter release by inhibiting the assembly of trans-SNARE complexes between transport vesicles and the plasma membrane, via the displacement of munc18 from syntaxin-1. There are three splice variants of tomosyn designated m-tomosyn, b-tomosyn and s-tomosyn. Although b-tomosyn is ubiquitously expressed, s-tomosyn and m-tomosyn are expressed primarily in brain.
  • Cellular localizationCell Membrane and Cytoplasmic. Note: Cytoplasmic, and associated with vesicular membranes and the plasma membrane. Detected at synapses and on synaptic vesicles
  • Database links
  • Alternative names
    • FLJ30922 antibody
    • Lethal(2) giant larvae protein homolog 3 antibody
    • LGL3 antibody
    • LLGL3 antibody
    • MGC141942 antibody
    • MGC141968 antibody
    • Nbla04300 antibody
    • STXBP5 antibody
    • Syntaxin binding protein 5 antibody
    • Tomosyn 1 antibody
    see all

Anti-Tomosyn antibody images

  • Anti-Tomosyn antibody (ab41271) at 1 µg/ml + Brain (Rat) Tissue Lysate - normal tissue at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 128 kDa
    Observed band size : 128 kDa
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
    Tamosyn is phosphorylated on a number of serine and threonine residues (SwissProt), which may account for the banding pattern observed. ab41271 is targeted against all isoforms of the Tamosyn protein.
  • Immunohistochemistical detection of tomosyn using antibody (ab41271) on PFA perfusion fixed, free floating rat brain sections. Primary Antibody ab41271 was diluted 1/3000 and incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary antibody: goat anti-rabbit Alexa Fluor® 488 (1/1000). The antibody produced a diffuse staining in many brain areas, corresponding to the staining published by Fujita 1998 (Nature). The submitted image is of hippocampal staining.

    See Abreview

References for Anti-Tomosyn antibody (ab41271)

ab41271 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

Submitted Apr 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"