Overview

  • Product name
  • Description
    Rabbit polyclonal to Tomosyn
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity

    Predicted to work with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 850 - 950 of Human Tomosyn.

    (Peptide available as ab45227.)

  • Positive control
    • This antibody gave a positive signal in the following tissues lysates: Rat Brain, Mouse Brain and Human Brain

Properties

Applications

Our Abpromise guarantee covers the use of ab45228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).

Target

  • Relevance
    Tomosyn plays a regulatory role in calcium-dependent exocytosis and neurotransmitter release by inhibiting the assembly of trans-SNARE complexes between transport vesicles and the plasma membrane, via the displacement of munc18 from syntaxin-1. There are three splice variants of tomosyn designated m-tomosyn, b-tomosyn and s-tomosyn. Although b-tomosyn is ubiquitously expressed, s-tomosyn and m-tomosyn are expressed primarily in brain.
  • Cellular localization
    Cell Membrane and Cytoplasmic. Note: Cytoplasmic, and associated with vesicular membranes and the plasma membrane. Detected at synapses and on synaptic vesicles
  • Database links
  • Alternative names
    • FLJ30922 antibody
    • Lethal(2) giant larvae protein homolog 3 antibody
    • LGL3 antibody
    • LLGL3 antibody
    • MGC141942 antibody
    • MGC141968 antibody
    • Nbla04300 antibody
    • STXBP5 antibody
    • Syntaxin binding protein 5 antibody
    • Tomosyn 1 antibody
    see all

Images

  • All lanes : Anti-Tomosyn antibody (ab45228) at 1 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Human) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 128 kDa
    Observed band size : 128 kDa
    Additional bands at : 30 kDa,52 kDa,60 kDa. We are unsure as to the identity of these extra bands.

References

ab45228 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab45228.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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