Overview

  • Product name
  • Description
    Rabbit polyclonal to TOP1MT
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen, corresponding to amino acids 175-310 of Human TOP1MT.

  • Positive control
    • Human cerebral cortex tissue; A431 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval method is Heat Induced Epitope Retrieval (HIER) in retrieval buffer pH 6, using a pressure boiler.
ICC/IF Use a concentration of 1 - 4 µg/ml. Recommended conditions: Fixation/Permeabilization: PFA/Triton X-100

Target

  • Function
    Relieves DNA strain that arise during duplication of mitochondrial DNA.
  • Tissue specificity
    Ubiquitous; highest in skeletal muscle, heart, brain and fetal liver.
  • Sequence similarities
    Belongs to the eukaryotic type I topoisomerase family.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2900052H09Rik antibody
    • DNA topoisomerase I antibody
    • DNA topoisomerase I, mitochondrial antibody
    • mitochondrial antibody
    • TOP1M_HUMAN antibody
    • TOP1mt antibody
    • Topoisomerase I mitochondrial antibody
    see all

Images

  • ab121145 at 1/100 dilution staining TOP1MT in Paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry, showing strong nuclear and cytoplasmic positivity in neuronal cells. .
  • ab121145 at 4 µg/ml staining TOP1MT in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).
    Note: positivity in mitochondria.

References

ab121145 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab121145.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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