Overview

  • Product nameAnti-TopBP1 antibody
    See all TopBP1 primary antibodies
  • Description
    Rabbit polyclonal to TopBP1
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human, Xenopus laevis
    Predicted to work with: Rabbit, Horse, Cow, Dog, Macaque Monkey, Gorilla, Chinese Hamster
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 800 - 900 of Human TopBP1.

  • Positive control
    • This antibody gave a positive signal in Human Brain tissue lysate as well as the following whole cell lysates: HEK293; HeLa; MCF7; U20S.

Properties

Applications

Our Abpromise guarantee covers the use of ab105109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 171 kDa).

Target

  • FunctionRequired for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double-stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR.
  • Tissue specificityHighly expressed in heart, brain, placenta, lung and kidney.
  • Sequence similaritiesContains 8 BRCT domains.
  • Post-translational
    modifications
    Phosphorylated on serine and threonine residues in response to X-ray irradiation.
    Ubiquitinated and degraded by the proteasome. X-ray irradiation reduces ubiquitination.
  • Cellular localizationNucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Chromosome. Detected on unpaired autosomes in meiotic prophase cells. Detected on X and Y chromosomes during later stages of prophase. Colocalizes with ATR and H2AFX at unsynapsed chromosome cores during prophase (By similarity). Has a uniform nuclear distribution during G phase. Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation. Localizes to sites of DNA damage in a H2AX- independent manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA topoisomerase 2 binding protein 1 antibody
    • DNA topoisomerase 2-binding protein 1 antibody
    • DNA topoisomerase II binding protein 1 antibody
    • DNA topoisomerase II binding protein antibody
    • DNA topoisomerase II-beta-binding protein 1 antibody
    • DNA topoisomerase II-binding protein 1 antibody
    • DNA topoisomerase IIbeta binding protein 1 antibody
    • Hypothetical protein KIAA0259 [Fragment] antibody
    • KIAA0259 antibody
    • TOP2BP1 antibody
    • TOPB1_HUMAN antibody
    • TOPBP 1 antibody
    • TopBP1 antibody
    • Topoisomerase (DNA) II binding protein 1 antibody
    • Topoisomerase II binding protein 1 antibody
    see all

Anti-TopBP1 antibody images

  • All lanes : Anti-TopBP1 antibody (ab105109) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 5 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 171 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 120 kDa,42 kDa,56 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • All lanes : Anti-TopBP1 antibody (ab105109) at 0.5 µg/ml

    Lane 1 : Mouse ES whole cell lysate
    Lane 2 : Mouse ES whole cell lysate

    Lysates/proteins at 200 µg per lane.

    Secondary
    HRP Goat anti-rabbit IgG polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 171 kDa
    Observed band size : 200 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 145 kDa (possible non-specific binding),85 kDa (possible non-specific binding).

    Exposure time : 30 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-TopBP1 antibody (ab105109)

This product has been referenced in:
  • Vasileva A  et al. The DNA damage checkpoint protein RAD9A is essential for male meiosis in the mouse. J Cell Sci 126:3927-38 (2013). Read more (PubMed: 23788429) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Xenopus laevis Cell (Nuclei generated in oocyte extract)
Specification Nuclei generated in oocyte extract
Fixative Formaldehyde
Permeabilization Yes - PBS-0.1% NP40
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Username

Dr. Andrew Lane

Verified customer

Submitted Apr 12 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Xenopus laevis Recombinant protein (E. coli lysate w. recombinant X. laevis GST-TOPBP1)
Loading amount 30 µg
Specification E. coli lysate w. recombinant X. laevis GST-TOPBP1
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Dr. Andrew Lane

Verified customer

Submitted Apr 12 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Embryonic stem cell)
Loading amount 200 µg
Specification Embryonic stem cell
Gel Running Conditions Reduced Denaturing (3-8% tris-acetate gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 27 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"