Anti-Topoisomerase I antibody (ab28432)
Key features and details
- Rabbit polyclonal to Topoisomerase I
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Topoisomerase I antibody
See all Topoisomerase I primary antibodies -
Description
Rabbit polyclonal to Topoisomerase I -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length Topoisomerase I protein (Human).
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Positive control
- HeLa nuclear extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.9
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Ab28432 is affinity purified. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28432 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 1 µg/ml.
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Notes |
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WB
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 1 µg/ml. |
Target
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Function
The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. -
Involvement in disease
Note=A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98. -
Sequence similarities
Belongs to the eukaryotic type I topoisomerase family. -
Post-translational
modificationsSumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment. -
Cellular localization
Nucleus > nucleolus. Nucleus > nucleoplasm. Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli. - Information by UniProt
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Database links
- Entrez Gene: 7150 Human
- Omim: 126420 Human
- SwissProt: P11387 Human
- Unigene: 472737 Human
- Unigene: 712686 Human
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Alternative names
- DNA topoisomerase 1 antibody
- DNA topoisomerase I antibody
- NUP98 fusion gene antibody
see all
Images
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ICC/IF image of ab28432 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28432, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Text
Text -
ICC/IF image of ab28432 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28432, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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Datasheet download
References (10)
ab28432 has been referenced in 10 publications.
- Meroni A et al. NEDDylated Cullin 3 mediates the adaptive response to topoisomerase 1 inhibitors. Sci Adv 8:eabq0648 (2022). PubMed: 36490343
- Jensen NF et al. Characterization of DNA topoisomerase I in three SN-38 resistant human colon cancer cell lines reveals a new pair of resistance-associated mutations. J Exp Clin Cancer Res 35:56 (2016). PubMed: 27029323
- Xia Q et al. Characterization of the transcriptional machinery bound across the widely presumed type 2 diabetes causal variant, rs7903146, within TCF7L2. Eur J Hum Genet N/A:N/A (2014). Human . PubMed: 24667787
- Kiianitsa K & Maizels N Ultrasensitive isolation, identification and quantification of DNA-protein adducts by ELISA-based RADAR assay. Nucleic Acids Res 42:e108 (2014). PubMed: 24914050
- Kiianitsa K & Maizels N A rapid and sensitive assay for DNA-protein covalent complexes in living cells. Nucleic Acids Res 41:e104 (2013). WB ; Human . PubMed: 23519618
- Montariello D et al. p63 involvement in poly(ADP-ribose) polymerase 1 signaling of topoisomerase I-dependent DNA damage in carcinoma cells. Biochem Pharmacol 85:999-1006 (2013). Human . PubMed: 23376119
- Pfister TD et al. Development and validation of an immunoassay for quantification of topoisomerase I in solid tumor tissues. PLoS One 7:e50494 (2012). Human . PubMed: 23284638
- D'Onofrio G et al. Poly(ADP-ribose) polymerase signaling of topoisomerase 1-dependent DNA damage in carcinoma cells. Biochem Pharmacol 81:194-202 (2011). WB ; Human . PubMed: 20875401
- Pfister TD et al. Topoisomerase I levels in the NCI-60 cancer cell line panel determined by validated ELISA and microarray analysis and correlation with indenoisoquinoline sensitivity. Mol Cancer Ther 8:1878-84 (2009). ELISA ; Human . PubMed: 19584232
- Cohen AA et al. Dynamic proteomics of individual cancer cells in response to a drug. Science 322:1511-6 (2008). WB ; Human . PubMed: 19023046