Overview

  • Product nameAnti-Topoisomerase I antibody
    See all Topoisomerase I primary antibodies
  • Description
    Rabbit polyclonal to Topoisomerase I
  • SpecificityAb28432 recognises Topoisomerase I.
  • Tested applicationsSuitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Topoisomerase I protein (Human).

  • Positive control
    • HeLa nuclear extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab28432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/5000. Predicted molecular weight: 91 kDa.
ICC/IF Use a concentration of 1 µg/ml.
ELISA 1/500.

Target

  • FunctionThe reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
  • Involvement in diseaseNote=A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98.
  • Sequence similaritiesBelongs to the eukaryotic type I topoisomerase family.
  • Post-translational
    modifications
    Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment.
  • Cellular localizationNucleus > nucleolus. Nucleus > nucleoplasm. Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA topoisomerase 1 antibody
    • DNA topoisomerase I antibody
    • NUP98 fusion gene antibody
    • TOP 1 antibody
    • TOP I antibody
    • TOP1 antibody
    • TOP1_HUMAN antibody
    • TOPI antibody
    • Topoisomerase (DNA) I antibody
    • Topoisomerase 1 antibody
    • Topoisomerase1 antibody
    • TopoisomeraseI antibody
    • Type I DNA topoisomerase antibody
    see all

Anti-Topoisomerase I antibody images

  • ICC/IF image of ab28432 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28432, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
    Text
    Text
  • ICC/IF image of ab28432 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28432, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Topoisomerase I antibody (ab28432)

This product has been referenced in:
  • Xia Q  et al. Characterization of the transcriptional machinery bound across the widely presumed type 2 diabetes causal variant, rs7903146, within TCF7L2. Eur J Hum Genet N/A:N/A (2014). Human . Read more (PubMed: 24667787) »
  • Kiianitsa K & Maizels N Ultrasensitive isolation, identification and quantification of DNA-protein adducts by ELISA-based RADAR assay. Nucleic Acids Res 42:e108 (2014). Read more (PubMed: 24914050) »

See all 8 Publications for this product

Product Wall

We have not tested ab28432 for mouse reactivity, but is likely that most topoisomerase antibodies, including ab28432, that react with human samples will react with mouse samples, given the 96% identity shared by the two proteins. This is based on align...

Read More

I have attached the Certificate of Compliance you requested. Please let me know if you need anything further.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"