Overview

  • Product nameAnti-Topoisomerase I antibody
    See all Topoisomerase I primary antibodies
  • Description
    Mouse monoclonal to Topoisomerase I
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: QRLEEQLMKL EVQATDREEN KQIALGTSKL NYLDPRITVA WCKKWGVPIE KIYNKTQREK FAWAIDMADE DYEF, corresponding to amino acids 692-766 of Human Topoisomerase I

Properties

Applications

Our Abpromise guarantee covers the use of ab58313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesIHC-P: Use at a concentration of 1-5 µg/ml.
    WB: Use at a concentration of 1 - 5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThe reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
    • Involvement in diseaseNote=A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98.
    • Sequence similaritiesBelongs to the eukaryotic type I topoisomerase family.
    • Post-translational
      modifications
      Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment.
    • Cellular localizationNucleus > nucleolus. Nucleus > nucleoplasm. Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli.
    • Information by UniProt
    • Database links
    • Alternative names
      • DNA topoisomerase 1 antibody
      • DNA topoisomerase I antibody
      • NUP98 fusion gene antibody
      • TOP 1 antibody
      • TOP I antibody
      • TOP1 antibody
      • TOP1_HUMAN antibody
      • TOPI antibody
      • Topoisomerase (DNA) I antibody
      • Topoisomerase 1 antibody
      • Topoisomerase1 antibody
      • TopoisomeraseI antibody
      • Type I DNA topoisomerase antibody
      see all

    Anti-Topoisomerase I antibody images

    • Topoisomerase I antibody (ab58313) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human hepatocellular carcinoma.
    • Western blot against tagged recombinant protein immunogen using ab58313 Topoisomerase I antibody at 1ug/ml. Predicted band size of immunogen is 34 kDa

    References for Anti-Topoisomerase I antibody (ab58313)

    This product has been referenced in:
    • Arnò B  et al. Replacement of the human topoisomerase linker domain with the plasmodial counterpart renders the enzyme camptothecin resistant. PLoS One 8:e68404 (2013). Read more (PubMed: 23844196) »
    • Tesauro C  et al. Molecular mechanism of the camptothecin resistance of Glu710Gly topoisomerase IB mutant analyzed in vitro and in silico. Mol Cancer 12:100 (2013). WB . Read more (PubMed: 24004603) »

    See all 3 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% TritonX100 in PBS
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Jul 14 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"