Anti-Topoisomerase II alpha antibody (ab74715)

Overview

  • Product name
    Anti-Topoisomerase II alpha antibody
    See all Topoisomerase II alpha primary antibodies
  • Description
    Rabbit polyclonal to Topoisomerase II alpha
  • Specificity
    ab74715 detects endogenous levels of total Topoisomerase II alpha protein.
  • Tested applications
    Suitable for: ELISA, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Topoisomerase II alpha (N terminal). Immunogen is within the range of aa 1-50.
    Database link: P11388

  • Positive control
    • Human liver carcinoma tissue; extracts from HT-29 cells; A549 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab74715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
WB 1/500 - 1/1000. Predicted molecular weight: 174 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/500.

Target

  • Function
    Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.
  • Sequence similarities
    Belongs to the type II topoisomerase family.
  • Post-translational
    modifications
    Phosphorylation has no effect on catalytic activity.
  • Cellular localization
    Cytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • alpha isozyme antibody
    • ATP hydrolyzing DNA topoisomerase II alfa antibody
    • DNA gyrase antibody
    • DNA topoisomerase (ATP hydrolyzing) antibody
    • DNA topoisomerase 2 alpha antibody
    • DNA topoisomerase 2-alpha antibody
    • DNA topoisomerase II 170 kD antibody
    • DNA topoisomerase II alpha isozyme antibody
    • DNA topoisomerase II antibody
    • DNA Topoisomerase2 antibody
    • TOP 2A antibody
    • TOP2 antibody
    • TOP2A antibody
    • TOP2A_HUMAN antibody
    • Topoisomerase DNA II alpha 170kDa antibody
    • TP2A antibody
    see all

Images

  • All lanes : Anti-Topoisomerase II alpha antibody (ab74715) at 1/500 dilution

    Lane 1 : extracts from HT-29 cells
    Lane 2 : extracts from HT-29 cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 174 kDa
    Observed band size : 174 kDa
  • Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using ab74715 at 1/50-1/100 dilution in the presence (+) or absence (-) of immunising peptide.
  • Immunofluorescence analysis of A549 cells, using ab74715 at 1/100-1/500 dilution in the presence (+) or absence (-) of immunising peptide.

References

This product has been referenced in:
  • Wang S  et al. Effect and mechanism of resveratrol on drug resistance in human bladder cancer cells. Mol Med Rep 15:1179-1187 (2017). Read more (PubMed: 28098863) »
  • Marthandan S  et al. Conserved Senescence Associated Genes and Pathways in Primary Human Fibroblasts Detected by RNA-Seq. PLoS One 11:e0154531 (2016). WB . Read more (PubMed: 27140416) »

See all 4 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (4-12 Bis tris)
Sample
Human Cell lysate - whole cell (293T)
Specification
293T
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jul 14 2014

Thank you for your inquiry. The antibodies (ab6078, ab8609, ab20034, ab22683, ab32575, ab66511, ab74715) need an MSDS only for the Sodium Azide in the storage buffer. For ab66511 there is no MSDS neccessary at all. The MSDS can be found on the da...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Xenopus laevis Cell lysate - whole cell (meiotic egg extract)
Loading amount
25 µg
Specification
meiotic egg extract
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 28 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Xenopus laevis Cell (X. laevis cell free egg extract)
Specification
X. laevis cell free egg extract
Fixative
Formaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Username

Abcam user community

Verified customer

Submitted Oct 18 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up