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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity Tissue culture supernatant
Clone number EP2722
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500. Detects a band of approximately 174 kDa (predicted molecular weight: 174 kDa).
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/100 - 1/250.
Application notes Is unsuitable for Flow Cyt or IP.
Function Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.
Sequence similarities Belongs to the type II topoisomerase family.
Post-translational modifications Phosphorylation has no effect on catalytic activity.
Cellular localization Cytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm.
Information by UniProt
alpha isozyme antibody
ATP hydrolyzing DNA topoisomerase II alfa antibody
DNA gyrase antibody
Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722] images
Western blot - Topoisomerase II alpha (phospho S1106) antibody [EP2722] (ab75765)
All lanes : Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722] (ab75765) at 1/500 dilution Lane 1 : HeLa cell lysates, untreated Lane 2 : HeLa cell lysates, treated with AP Lysates/proteins at 10 µg per lane. Secondary goat anti-rabbit HRP at 1/2000 dilution Predicted band size : 174 kDa Observed band size : 174 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Topoisomerase II alpha (phospho S1106) antibody [EP2722] (ab75765)
ab75765 at 1/100 dilution staining Topoisomerase II alpha in colonic adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue
References for Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722] (ab75765)
This product has been referenced in:
et al. DNA damage response (DDR) induced by topoisomerase II poisons requires nuclear function of the small GTPase Rac. Biochim Biophys Acta 1833:3093-3103 (2013).
Read more (PubMed: 23999236) »
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"