Recombinant Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1054Y] to Topoisomerase II alpha (phospho T1343)
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y]
See all Topoisomerase II alpha primary antibodies -
Description
Rabbit monoclonal [EP1054Y] to Topoisomerase II alpha (phospho T1343) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa cell lysates. IHC-P: Human cervical carcinoma tissue. ICC/IF: HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1054Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/1000. Detects a band of approximately 174 kDa (predicted molecular weight: 174 kDa).
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ICC/IF |
1/50 - 1/100.
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Notes |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000. Detects a band of approximately 174 kDa (predicted molecular weight: 174 kDa). |
ICC/IF
1/50 - 1/100. |
Target
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Function
Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. -
Sequence similarities
Belongs to the type II topoisomerase family. -
Post-translational
modificationsPhosphorylation has no effect on catalytic activity. -
Cellular localization
Cytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm. - Information by UniProt
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Database links
- Entrez Gene: 7153 Human
- Omim: 126430 Human
- SwissProt: P11388 Human
- Unigene: 156346 Human
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Alternative names
- alpha isozyme antibody
- ATP hydrolyzing DNA topoisomerase II alfa antibody
- DNA gyrase antibody
see all
Images
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All lanes : Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853) at 1/1000 dilution
Lane 1 : HeLa cell lysate treated with AP
Lane 2 : HeLa cell lysate untreated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 174 kDa
Observed band size: 174 kDa -
ab52853 at 1/100 dilution staining Topoisomerase II alpha in paraffin embedded human cervical carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Topoisomerase II alpha with purified ab52853 at 1/50 dilution (4 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.14 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control: PBS instead of the primary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab52853 has not yet been referenced specifically in any publications.