Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)

Overview

  • Product nameAnti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y]
    See all Topoisomerase II alpha primary antibodies
  • Description
    Rabbit monoclonal [EP1054Y] to Topoisomerase II alpha (phospho T1343)
  • Specificityab52853 only detects Topoisomerase II alpha phosphorylated on Threonine 1343.
  • Tested applicationsSuitable for: IHC-P, WB, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic phospho-peptide corresponding to residues surrounding Thr 1343 of human Topoisomerase II alpha.

  • Positive control
    • WB: HeLa cell lysates. IHC-P: Human cervical carcinoma tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab52853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 174 kDa (predicted molecular weight: 174 kDa).
ICC 1/50 - 1/100.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionControl of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.
    • Sequence similaritiesBelongs to the type II topoisomerase family.
    • Post-translational
      modifications
      Phosphorylation has no effect on catalytic activity.
    • Cellular localizationCytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • alpha isozyme antibody
      • ATP hydrolyzing DNA topoisomerase II alfa antibody
      • DNA gyrase antibody
      • DNA topoisomerase (ATP hydrolyzing) antibody
      • DNA topoisomerase 2 alpha antibody
      • DNA topoisomerase 2-alpha antibody
      • DNA topoisomerase II 170 kD antibody
      • DNA topoisomerase II alpha isozyme antibody
      • DNA topoisomerase II antibody
      • DNA Topoisomerase2 antibody
      • TOP 2A antibody
      • TOP2 antibody
      • TOP2A antibody
      • TOP2A_HUMAN antibody
      • Topoisomerase DNA II alpha 170kDa antibody
      • TP2A antibody
      see all

    Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] images

    • All lanes : Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853) at 1/1000 dilution

      Lane 1 : HeLa cell lysate treated with AP
      Lane 2 : HeLa cell lysate untreated

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 174 kDa
      Observed band size : 174 kDa
    • ab52853 at 1/100 dilution staining Topoisomerase II alpha in paraffin embedded human cervical carcinoma tissue by Immunohistochemistry.

    References for Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)

    ab52853 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"