Anti-Topoisomerase II beta antibody (ab52769)

Overview

  • Product name
    Anti-Topoisomerase II beta antibody
    See all Topoisomerase II beta primary antibodies
  • Description
    Mouse polyclonal to Topoisomerase II beta
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Chicken
    Predicted to work with: Mouse, Rat, Dog, Human, Chimpanzee
  • Immunogen

    Synthetic peptide: LVNAADNKQR DKNMTCIKIS IDPESNIISI WNNGKGIPVV EHKVEKVYVP ALIFGQLLTS SNYDDDEKKV TGGRNGYGAK LCNIFSTKFT VETACKEYKH , corresponding to amino acids 115-215 of Chicken Topoisomerase II beta; which has been tagged

  • General notes

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 50% Glycerol, Whole serum
  • Purity
    Whole antiserum
  • Primary antibody notes
    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 183 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Target

  • Function
    Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Indirectly ivolved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
  • Sequence similarities
    Belongs to the type II topoisomerase family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen MLAA 44 antibody
    • beta isozyme antibody
    • DNA topoisomerase 2 beta antibody
    • DNA topoisomerase 2-beta antibody
    • DNA topoisomerase II 180 kD antibody
    • DNA topoisomerase II antibody
    • DNA topoisomerase II beta antibody
    • DNA topoisomerase II beta isozyme antibody
    • Top 2 antibody
    • TOP 2B antibody
    • TOP IIB antibody
    • Top2 beta antibody
    • TOP2B antibody
    • TOP2B_HUMAN antibody
    • Top2beta antibody
    • TOPIIB antibody
    • Topo II beta antibody
    • Topoisomerase (DNA) II beta 180kDa antibody
    • Topoisomerase (DNA) II beta antibody
    • Topoisomerase IIb antibody
    • U937 associated antigen antibody
    see all

Anti-Topoisomerase II beta antibody images

  • All lanes : Anti-Topoisomerase II beta antibody (ab52769) at 1/1000 dilution

    Lane 1 : Total protein extract from E coli with ~50ng to 100 ng of an irrelevant antigen
    Lane 2 : Total protein extract from E coli with ~50ng to 500ng of the antigen

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 183 kDa

References for Anti-Topoisomerase II beta antibody (ab52769)

ab52769 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (colon cancer cells)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
60 µg
Specification
colon cancer cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 10 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Blocking
Sample
Zebrafish Tissue lysate - other (heart)
Specification
heart
Username

Yonghe Ding

Verified customer

Submitted Dec 12 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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