Anti-Topoisomerase II beta antibody (ab52769)

Overview

  • Product nameAnti-Topoisomerase II beta antibody
    See all Topoisomerase II beta primary antibodies
  • Description
    Mouse polyclonal to Topoisomerase II beta
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Chicken
    Predicted to work with: Mouse, Rat, Dog, Human, Chimpanzee
  • Immunogen

    Synthetic peptide: LVNAADNKQR DKNMTCIKIS IDPESNIISI WNNGKGIPVV EHKVEKVYVP ALIFGQLLTS SNYDDDEKKV TGGRNGYGAK LCNIFSTKFT VETACKEYKH , corresponding to amino acids 115-215 of Chicken Topoisomerase II beta; which has been tagged

  • General notes

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • PurityWhole antiserum
  • Primary antibody notesThis antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 183 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Target

  • FunctionControl of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Indirectly ivolved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
  • Sequence similaritiesBelongs to the type II topoisomerase family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen MLAA 44 antibody
    • beta isozyme antibody
    • DNA topoisomerase 2 beta antibody
    • DNA topoisomerase 2-beta antibody
    • DNA topoisomerase II 180 kD antibody
    • DNA topoisomerase II antibody
    • DNA topoisomerase II beta antibody
    • DNA topoisomerase II beta isozyme antibody
    • Top 2 antibody
    • TOP 2B antibody
    • TOP IIB antibody
    • Top2 beta antibody
    • TOP2B antibody
    • TOP2B_HUMAN antibody
    • Top2beta antibody
    • TOPIIB antibody
    • Topo II beta antibody
    • Topoisomerase (DNA) II beta 180kDa antibody
    • Topoisomerase (DNA) II beta antibody
    • Topoisomerase IIb antibody
    • U937 associated antigen antibody
    see all

Anti-Topoisomerase II beta antibody images

  • All lanes : Anti-Topoisomerase II beta antibody (ab52769) at 1/1000 dilution

    Lane 1 : Total protein extract from E coli with ~50ng to 100 ng of an irrelevant antigen
    Lane 2 : Total protein extract from E coli with ~50ng to 500ng of the antigen

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 183 kDa

References for Anti-Topoisomerase II beta antibody (ab52769)

ab52769 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab52769.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"