Anti-Topoisomerase III alpha antibody (ab108493)

Overview

  • Product nameAnti-Topoisomerase III alpha antibody
    See all Topoisomerase III alpha primary antibodies
  • Description
    Rabbit polyclonal to Topoisomerase III alpha
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 900 - 1000 of Human Topoisomerase III alpha.

  • Positive control
    • This antibody gave a positive signal in both HeLa and HEK293 whole cell lysates. This antibody gave a positive result in IF in the following Methanol fixed cell line: HeLa.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 112 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionReduces the number of supercoils in a highly negatively supercoiled DNA. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells.
  • Tissue specificityHigh expression is found in testis, heart, skeletal muscle and pancreas.
  • Sequence similaritiesBelongs to the prokaryotic type I/III topoisomerase family.
    Contains 1 Toprim domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA topoisomerase 3 alpha antibody
    • DNA topoisomerase 3-alpha antibody
    • DNA topoisomerase III alpha antibody
    • TOP 3 antibody
    • TOP3 antibody
    • TOP3A antibody
    • TOP3A_HUMAN antibody
    • topo III-alpha antibody
    • topoisomerase (DNA) III alpha antibody
    • topoisomerase (DNA) III antibody
    • topoisomerase III alpha antibody
    • ZGRF7 antibody
    • zinc finger, GRF-type containing 7 antibody
    see all

Anti-Topoisomerase III alpha antibody images

  • All lanes : Anti-Topoisomerase III alpha antibody (ab108493) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 112 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 16 minutes
  • ab108493 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab108493 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Topoisomerase III alpha antibody (ab108493)

ab108493 has not yet been referenced specifically in any publications.

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