Overview

  • Product nameAnti-TOR1AIP1 antibody
    See all TOR1AIP1 primary antibodies
  • Description
    Rabbit polyclonal to TOR1AIP1
  • Tested applicationsSuitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 175 and 225 of human TOR1AIP1 (NP_056417.2).

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab86307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
IP
  • Application notesIP: Use at 2-5 µg/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 66 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionBinds to A- and B-type lamins. Possible role in membrane attachment and assembly of the nuclear lamina.
    • Sequence similaritiesBelongs to the TOR1AIP family.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationNucleus inner membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Lamin-associated protein 1B antibody
      • Lamina associated polypeptide 1B antibody
      • Lamina associated polypeptide 1C antibody
      • LAP1 antibody
      • LAP1B antibody
      • TOIP1_HUMAN antibody
      • TOR1AIP1 antibody
      • Torsin A interacting protein 1 antibody
      • Torsin-1A-interacting protein 1 antibody
      see all

    Anti-TOR1AIP1 antibody images

    • All lanes : Anti-TOR1AIP1 antibody (ab86307) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 66 kDa
      Observed band size : 66 kDa


      Exposure time : 30 seconds
    • Detection of TOR1AIP1 by Western Blot of Immunprecipitate.
      ab86307, at 1 µg/ml, staining TOR1AIP1 in HeLa whole cell lysate immunoprecipitated using ab86307 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with an exposure time of 10 seconds.

    References for Anti-TOR1AIP1 antibody (ab86307)

    ab86307 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 2% · Temperature: 23°C
    Sample Human Cell (HeLa)
    Specification HeLa
    Permeabilization Yes - CSK buffer, 5 min at RT after fixing
    Fixative Paraformaldehyde
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    Verified customer

    Submitted Sep 20 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"