Overview

  • Product nameAnti-TORC1 antibody [EPR3382]See all TORC1 primary antibodies ...
  • Description
    Rabbit monoclonal [EPR3382] to TORC1
  • Tested applicationsWB, ICC, Flow Cyt more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human MECT1.

  • Positive control
    • Fetal brain and C6 cell lysates
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab92477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/1000 - 1/5000. Detects a band of approximately 78 kDa (predicted molecular weight: 67 kDa).
ICC 1/50 - 1/100.
Flow Cyt 1/1000 - 1/10000.
  • Application notesIs unsuitable for IHC-P or IP.
  • Target

    • FunctionTranscriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). In the hippocampus, involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses. May be required for dendritic growth of developing cortical neurons.
    • Tissue specificityHighly expressed in adult and fetal brain. Located to specific regions such as the prefrontal cortex and cerebellum. Very low expression in other tissues such as heart, spleen, lung, skeletal muscle, salivary gland, ovary and kidney.
    • Involvement in diseaseA chromosomal aberration involving CRTC1 is found in mucoepidermoid carcinomas, benign Warthin tumors and clear cell hidradenomas. Translocation t(11;19)(q21;p13) with MAML2. The fusion protein consists of the N-terminus of CRTC1 joined to the C-terminus of MAML2. The reciprocal fusion protein consisting of the N-terminus of MAML2 joined to the C-terminus of CRTC1 has been detected in a small number of mucoepidermoid carcinomas.
    • Sequence similaritiesBelongs to the TORC family.
    • Post-translational
      modifications
      Phosphorylation/dephosphorylation states of Ser-151 are required for regulating transduction of CREB activity. TORCs are inactive when phosphorylated, and active when dephosphorylated at this site. This primary site of phosphorylation is mediated by SIKs (SIK1 and SIK2), is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs by LKB1.
    • Cellular localizationCytoplasm. Nucleus. Cytoplasmic when phosphorylated by SIK or AMPK and when sequestered by 14-3-3 proteins (By similarity). Translocated to the nucleus on Ser-151 dephosphorylation, instigated by a number of factors including calcium ion and cAMP levels.
    • Target information above from: UniProt accession Q6UUV9 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • KIAA0616 antibody
      • CREB regulated transcription coactivator 1 antibody
      • CREB-regulated transcription coactivator 1 antibody
      • CRTC1 antibody
      • CRTC1_HUMAN antibody
      • FLJ14027 antibody
      • KIAA0616 antibody
      • MECT 1 antibody
      • Mucoepidermoid carcinoma translocated 1 antibody
      • Mucoepidermoid carcinoma translocated protein 1 antibody
      • TORC-1 antibody
      • TORC1 antibody
      • Transducer of CREB protein 1 antibody
      • Transducer of regulated cAMP response element binding protein 1 antibody
      • Transducer of regulated cAMP response element-binding protein (CREB) 1 antibody
      • Transducer of regulated cAMP response element-binding protein 1 antibody
      • WAMTP1 antibody
      see all

    Anti-TORC1 antibody [EPR3382] images

    • Overlay histogram showing SHSY-5Y cells stained with ab92477 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92477, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • All lanes : Anti-TORC1 antibody [EPR3382] (ab92477) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : C6 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit antibody at 1/2000 dilution

      Predicted band size : 67 kDa
      Observed band size : 78 kDa (why is the actual band size different from the predicted?)

    References for Anti-TORC1 antibody [EPR3382] (ab92477)

    ab92477 has not yet been referenced specifically in any publications.

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