Overview

  • Product nameAnti-TORC2 antibody [EPR3384(2)]
    See all TORC2 primary antibodies
  • Description
    Rabbit monoclonal [EPR3384(2)] to TORC2
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in the C-terminal of Human Torc2.

  • Positive control
    • 293T, Raji, and A673 cell lysates; Human tonsil tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab109081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 73 kDa.
IP 1/100.
IHC-P 1/100 - 1/250. Antigen retrieval is recommended.
  • Application notesIs unsuitable for Flow Cyt or ICC.
  • Target

    • FunctionTranscriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).
    • Tissue specificityMost abundantly expressed in the thymus. Present in both B and T lymphocytes. Highly expressed in HEK293T cells and in insulinomas. High levels also in spleen, ovary, muscle and lung, with highest levels in muscle. Lower levels found in brain, colon, heart, kidney, prostate, small intestine and stomach. Weak expression in liver and pancreas.
    • Sequence similaritiesBelongs to the TORC family.
    • Post-translational
      modifications
      Phosphorylation/dephosphorylation states of Ser-171 are required for regulating transduction of CREB activity. TORCs are inactive when phosphorylated, and active when dephosphorylated at this site. This primary site of phosphorylation, is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs by LKB1. Both insulin and AMPK increase this phosphorylation, of TORC2 while glucagon suppresses it.
    • Cellular localizationCytoplasm. Nucleus. Translocated from the nucleus to the cytoplasm on interaction of the phosphorylated form with 14-3-3 protein. In response to cAMP levels and glucagon, relocated to the nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • CREB regulated transcription coactivator 2 antibody
      • CREB-regulated transcription coactivator 2 antibody
      • CRTC2 antibody
      • CRTC2_HUMAN antibody
      • RP11-422P24.6 antibody
      • TORC-2 antibody
      • torc2 antibody
      • Transducer of CREB protein 2 antibody
      • Transducer of regulated cAMP response element-binding protein antibody
      • Transducer of regulated cAMP response element-binding protein (CREB) 2 antibody
      • Transducer of regulated cAMP response element-binding protein 2 antibody
      • Transducer of regulated CREB protein 2 antibody
      see all

    Anti-TORC2 antibody [EPR3384(2)] images

    • Anti-TORC2 antibody [EPR3384(2)] (ab109081) at 1/5000 dilution + Ins-1, rat insulinoma cell line, nuclear cell lysate at 1200000 cells

      Secondary
      HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 73 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)


      Exposure time : 5 minutes

      This image is courtesy of an Abreview submitted by Javier Triñanes

      See Abreview

    • All lanes : Anti-TORC2 antibody [EPR3384(2)] (ab109081) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : A673 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 73 kDa
    • ab109081 at 1/100 dilution staining Torc2 in Human tonsil by Immunohistochemistry, Paraffin-embedded tissue.

    References for Anti-TORC2 antibody [EPR3384(2)] (ab109081)

    This product has been referenced in:

    See all 2 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 1.2e+006 cells
    Gel Running Conditions Reduced Denaturing
    Sample Rat Cell lysate - nuclear (Ins-1, a rat insulinoma cell line)
    Specification Ins-1, a rat insulinoma cell line
    Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Dr. Javier Triñanes

    Verified customer

    Submitted Dec 23 2013

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (liver)
    Specification liver
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated
    Username

    Dr. Sandra Guilmeau

    Verified customer

    Submitted Jan 09 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"