Anti-TORC2 antibody [EPR3384(2)] (ab109081)


  • Product name
    Anti-TORC2 antibody [EPR3384(2)]
    See all TORC2 primary antibodies
  • Description
    Rabbit monoclonal [EPR3384(2)] to TORC2
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in the C-terminal of Human Torc2.

  • Positive control
    • 293T, Raji, and A673 cell lysates; Human tonsil tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents


Associated products


Our Abpromise guarantee covers the use of ab109081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 73 kDa.
IP 1/100.
IHC-P 1/100 - 1/250. Antigen retrieval is recommended.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function
      Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).
    • Tissue specificity
      Most abundantly expressed in the thymus. Present in both B and T lymphocytes. Highly expressed in HEK293T cells and in insulinomas. High levels also in spleen, ovary, muscle and lung, with highest levels in muscle. Lower levels found in brain, colon, heart, kidney, prostate, small intestine and stomach. Weak expression in liver and pancreas.
    • Sequence similarities
      Belongs to the TORC family.
    • Post-translational
      Phosphorylation/dephosphorylation states of Ser-171 are required for regulating transduction of CREB activity. TORCs are inactive when phosphorylated, and active when dephosphorylated at this site. This primary site of phosphorylation, is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs by LKB1. Both insulin and AMPK increase this phosphorylation, of TORC2 while glucagon suppresses it.
    • Cellular localization
      Cytoplasm. Nucleus. Translocated from the nucleus to the cytoplasm on interaction of the phosphorylated form with 14-3-3 protein. In response to cAMP levels and glucagon, relocated to the nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • CREB regulated transcription coactivator 2 antibody
      • CREB-regulated transcription coactivator 2 antibody
      • CRTC2 antibody
      • CRTC2_HUMAN antibody
      • RP11-422P24.6 antibody
      • TORC-2 antibody
      • torc2 antibody
      • Transducer of CREB protein 2 antibody
      • Transducer of regulated cAMP response element-binding protein antibody
      • Transducer of regulated cAMP response element-binding protein (CREB) 2 antibody
      • Transducer of regulated cAMP response element-binding protein 2 antibody
      • Transducer of regulated CREB protein 2 antibody
      see all


    • Anti-TORC2 antibody [EPR3384(2)] (ab109081) at 1/5000 dilution + Ins-1, rat insulinoma cell line, nuclear cell lysate at 1200000 cells

      HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 73 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)

      Exposure time : 5 minutes

      This image is courtesy of an Abreview submitted by Javier Triñanes

      See Abreview

    • All lanes : Anti-TORC2 antibody [EPR3384(2)] (ab109081) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : A673 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 73 kDa
    • ab109081 at 1/100 dilution staining Torc2 in Human tonsil by Immunohistochemistry, Paraffin-embedded tissue.


    This product has been referenced in:
    • Akhmedov D  et al. Gs-DREADD Knock-In Mice for Tissue-Specific, Temporal Stimulation of Cyclic AMP Signaling. Mol Cell Biol 37:N/A (2017). WB . Read more (PubMed: 28167604) »
    • Tian X  et al. CRTC2 enhances HBV transcription and replication by inducing PGC1a expression. Virol J 11:30 (2014). WB . Read more (PubMed: 24529027) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Western blot
    Loading amount
    1.2e+006 cells
    Gel Running Conditions
    Reduced Denaturing
    Rat Cell lysate - nuclear (Ins-1, a rat insulinoma cell line)
    Ins-1, a rat insulinoma cell line
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

    Dr. Javier Triñanes

    Verified customer

    Submitted Dec 23 2013

    Abcam has not validated the combination of species/application used in this Abreview.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Mouse Tissue sections (liver)
    Antigen retrieval step
    Heat mediated

    Dr. Sandra Guilmeau

    Verified customer

    Submitted Jan 09 2012


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