Overview

  • Product nameAnti-Torsin A antibody [EP2569Y]
    See all Torsin A primary antibodies
  • Description
    Rabbit monoclonal [EP2569Y] to Torsin A
  • Tested applicationsSuitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues near the C terminal of human Torsin A

  • Positive control
    • SH-SY5Y and 293T cell lysates. Permeabilized SH-SY5Y cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab76133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).
Flow Cyt 1/150. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for ICC,IHC-P or IP.
  • Target

    Anti-Torsin A antibody [EP2569Y] images

    • All lanes : Anti-Torsin A antibody [EP2569Y] (ab76133) at 1/1000 dilution

      Lane 1 : SH-SY5Y cell lysates
      Lane 2 : 293T cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 37 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 125 kDa,150 kDa. We are unsure as to the identity of these extra bands.
    • Flow cytometric analysis of permeabilized SH-SY5Y cells using ab76133 at 1/150 dilution (red) or a rabbit IgG (negative) (green).

    References for Anti-Torsin A antibody [EP2569Y] (ab76133)

    ab76133 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76133.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"