Recombinant
RabMAb

Anti-Torsin A antibody [EP2569Y] (ab76133)

Overview

  • Product name
    Anti-Torsin A antibody [EP2569Y]
    See all Torsin A primary antibodies
  • Description
    Rabbit monoclonal [EP2569Y] to Torsin A
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues near the C terminal of human Torsin A

  • Positive control
    • SH-SY5Y and 293T cell lysates. Permeabilized SH-SY5Y cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab76133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).
Flow Cyt 1/150. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    Images

    • All lanes : Anti-Torsin A antibody [EP2569Y] (ab76133) at 1/1000 dilution

      Lane 1 : SH-SY5Y cell lysates
      Lane 2 : 293T cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 37 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 125 kDa,150 kDa. We are unsure as to the identity of these extra bands.
    • Flow cytometric analysis of permeabilized SH-SY5Y cells using ab76133 at 1/150 dilution (red) or a rabbit IgG (negative) (green).

    References

    ab76133 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab76133.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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