Total OXPHOS Human WB Antibody Cocktail (ab110411)

Overview

  • Product name
    Total OXPHOS Human WB Antibody Cocktail
  • Assay type
    Quantitative
  • Species reactivity
    Reacts with: Human
  • Product overview

    ab110411  contains 5 mAbs, one each against Complex I subunit NDUFB8 (ab110242), Complex II subunit 30kDa (ab14714), Complex III subunit Core 2 (ab14745), Complex IV subunit II (ab110258), and ATP synthase subunit alpha (ab14748) as an optimized premixed cocktail. The cocktail is suitable for Western Blotting analysis of the relative levels of the 5 OXPHOS complexes in mitochondrial preparations from human sources. Human heart mitochondria (50 µg) are also included as a Western Blotting control.


    Altered levels of assembly can arise from mutations in individual subunits, mutations in assembly factors for the complex(es), mtDNA depletion or as a result of physiological and or pathological changes e.g. hormone treatment, exercise, diet or oxidative stress.


    The mAbs in the cocktail were chosen because they are against a subunit that is labile when its complex is not assembled. Moreover, the combination is readily resolved in SDS-PAGE when the appropriate gel conditions are used (see protocols).


    The Western blot cocktail is supplied at a concentration of 1.5 mg/mL.


    The five antibodies provided in the cocktail are all mouse monoclonal antibodies.


    We recommend using a high pH CAPS / PVDF transfer protocol when using this antibody for Western blot. Please contact us for more details. 


     

  • Notes

    Shipped  on Dry Ice.

  • Tested applications
    Suitable for: WBmore details

Properties

  • Alternative names
    • OXPHOS cocktail

Applications

Our Abpromise guarantee covers the use of ab110411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.

Recommended starting dilutions is 1:200 - 1:1000. However, optimal diltuion should be established by the end-user.

We recommend using a high pH CAPS / PVDF transfer protocol when using this antibody for Western blot. Please contact us for more details.

Total OXPHOS Human WB Antibody Cocktail images

  • This image is courtesy of an anonymous Abreview

    Gel: Non-reduced Denaturing (4-12%)

    Lanes 1-3: contain 3 individual Human B cell lymphoma samples 50 μg loaded per lane.

    Blocked for 1 hour with 5% Milk at room temperature.

    ab110411 used as per instructions.

    Secondary: HRP-conjugated anti-mouse IgG uesd at 1/300

    Detection method: ECL+ 2 minutes exposure.

     

  • Each lane contains 10 µg of mitochondria isolated from MRC5 fibroblasts.
    ab110411(MS601) was used to label lane 6, and the individual antibodies
    comprising the cocktail were used for lanes 1-5.

References for Total OXPHOS Human WB Antibody Cocktail (ab110411)

This product has been referenced in:
  • Nafisinia M  et al. Whole Exome Sequencing Identifies the Genetic Basis of Late-Onset Leigh Syndrome in a Patient with MRI but Little Biochemical Evidence of a Mitochondrial Disorder. JIMD Rep 32:117-124 (2017). WB ; Human . Read more (PubMed: 27344648) »
  • Bhattacharya D  et al. Decreased transcriptional corepressor p107 is associated with exercise-induced mitochondrial biogenesis in human skeletal muscle. Physiol Rep 5:N/A (2017). WB ; Human . Read more (PubMed: 28270591) »

See all 120 Publications for this product

Product Wall

Thank you for contacting us.

The answer is yes, you can use reducing reagent in the sample but we do not recommend boiling samples since it will cause signal reducing of several bands.

I hope this information is helpful to you. Pl...

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Western

Excellent Excellent 5/5 (Ease of Use)
Abreviews
MitoProfile Total OXPHOS
40 ug total protein from human heart tissue whole lysate
12% tris-glycine gel
5% milk-TBST block, 1 h, RT
primary of 1:750 in 5% milk for 16 h at 4C
secondary of 1:1250 in 5% milk for 1 h at RT
exposure varied based on band
excellent ease of use - 5/5
image attached
Username

Abcam user community

Verified customer

Submitted Oct 29 2014

Thank you for your enquiry.

I am pleased to help answer your questions:

1. what do you mean by labile protein for the differents complexes? are they degraded or found in the cytoplasm?

Several of the proteins are degrad...

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Thank you for your enquiry. It has taken some time to confirm all the details for you, and I appreciate your patience.

I am sorry to confirm that MS602 has been discontinued and we do not have anymore in stock.

Although that produ...

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Total OXPHOS antibody for western blotting

Excellent Excellent 5/5 (Ease of Use)
Abreviews
human fibroblast whole lysate used to detect OXPHOS
Username

Abcam user community

Verified customer

Submitted Jul 10 2017



The link below shows the genes in the mitochondrial DNA. This is a very comprehensive review.

http://www.nature.com/nrg/journal/v13/n12/full/nrg3275.html

There are 37 mitochondrial DNA (mtDNA)- encoded genes which include se...

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Total OXPHOS antibody cocktail

Excellent Excellent 5/5 (Ease of Use)
Abreviews
In this experiment we used isolated mitochondria from human HCT116 colon cancer cell line. The proteins from the mitochondrial lysate were seperated on a 13% SDS gel. The total OXPHOS human WB antibody kit was diluted 1:1000 in 2% milk. We already obtained strong signals of the complexes V and I after 10 sec. After 1 min (attached figure) we got signals from all five complexes.

The kit is easy to use and gives reproducibly results.
Username

Mr. Christian Marx

Verified customer

Submitted Aug 21 2013


In-house we test this cocktail using isolated mitochondra, but other users have published data using whole cell or whole tissue homogenates. We have these publications listed on the datasheet-

http://www.abcam.com/MitoProfilereg-Total-OXP...

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Thank you for your reply.

I can understand why you find the nomenclature a bit confusing and we will certainly strive in the future to make the nomenclature on our datasheets more consistent by ensuring that the full name is added to the targ...

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Thank you for your inquiry.

There is a representative protein from each of the 5 OXPHOS complexes in this mouse monoclonal antibody cocktail (ab110411, MS601).

I can confirm that one protein, COX-II (Uniprot P00403) is mtDNA encoded...

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1-10 of 26 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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