ab110411 contains 5 mAbs, one each against Complex I subunit NDUFB8 (ab110242), Complex II subunit 30kDa (ab14714), Complex III subunit Core 2 (ab14745), Complex IV subunit II (ab110258), and ATP synthase subunit alpha (ab14748) as an optimized premixed cocktail. The cocktail is suitable for Western Blotting analysis of the relative levels of the 5 OXPHOS complexes in mitochondrial preparations from human sources. Human heart mitochondria (50 µg) are also included as a Western Blotting control.
Altered levels of assembly can arise from mutations in individual subunits, mutations in assembly factors for the complex(es), mtDNA depletion or as a result of physiological and or pathological changes e.g. hormone treatment, exercise, diet or oxidative stress.
The mAbs in the cocktail were chosen because they are against a subunit that is labile when its complex is not assembled. Moreover, the combination is readily resolved in SDS-PAGE when the appropriate gel conditions are used (see protocols).
The Western blot cocktail is supplied at a concentration of 1.5 mg/mL.
The five antibodies provided in the cocktail are all mouse monoclonal antibodies.
Shipped on Dry Ice.
Store cocktail at 4°C and control at -80°C.
|Components||360 µg||720 µg|
|Human Heart Mitochondria Western Blot Control||1 x 50µg||1 x 50µg|
|Premix Cocktail of 5 monoclonal antibodies||1 x 360µg||1 x 720µg|
Our Abpromise guarantee covers the use of ab110411 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.
Recommended starting dilutions is 1:200 - 1:1000. However, optimal diltuion should be established by the end-user.
This image is courtesy of an anonymous Abreview
Gel: Non-reduced Denaturing (4-12%)
Lanes 1-3: contain 3 individual Human B cell lymphoma samples 50 μg loaded per lane.
Blocked for 1 hour with 5% Milk at room temperature.
ab110411 used as per instructions.
Secondary: HRP-conjugated anti-mouse IgG uesd at 1/300
Detection method: ECL+ 2 minutes exposure.