Overview

  • Product name
  • Description
    Rabbit polyclonal to TOX1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 55-301 of Human TOX1 (Uniprot ID: O94900).

  • Positive control
    • THP1 and HL60 whole cell lysates; Human 59T xenograft tissue.
  • General notes
    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab155768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 58 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • All lanes : Anti-TOX1 antibody (ab155768) at 1/1000 dilution

    Lane 1 : THP1 whole cell lysate
    Lane 2 : HL60 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 58 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human 59T xenograft tissue labeling TOX1 with ab155768 at 1/500 dilution.

References

ab155768 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Naive thymocytes from C57BL/6)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
3e+006 cells
Specification
Naive thymocytes from C57BL/6
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

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Verified customer

Submitted Feb 14 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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