Anti-TOX antibody (ab155768)
Key features and details
- Rabbit polyclonal to TOX
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-TOX antibody
See all TOX primary antibodies -
Description
Rabbit polyclonal to TOX -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment corresponding to Human TOX aa 55-301.
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Positive control
- WB: THP1 and HL60 whole cell lysates; IHC-P: Rat and mouse lung tissue. Rat liver tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155768 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 58 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 58 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May play a role in regulating T-cell development. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9760 Human
- Entrez Gene: 252838 Mouse
- Entrez Gene: 362481 Rat
- Omim: 606863 Human
- SwissProt: O94900 Human
- SwissProt: Q66JW3 Mouse
- Unigene: 491805 Human
- Unigene: 634856 Human
see all -
Alternative names
- KIAA0808 antibody
- Thymocyte selection-associated high mobility group box antibody
- Thymocyte selection-associated high mobility group box protein TOX antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue labelling TOX with ab155768 at 1/500 dilution. Antigen retrieval was performed using citrate buffer pH 6 for 15 minutes.
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All lanes : Anti-TOX antibody (ab155768) at 1/1000 dilution
Lane 1 : THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 2 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG (HRP)
Predicted band size: 58 kDaThe observed bands are consistent with what has been described in PMID: 18195075.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue labelling TOX with ab155768 at 1/500 dilution. Antigen retrieval was performed using citrate buffer pH 6 for 15 minutes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue labelling TOX with ab155768 at 1/500 dilution. Antigen retrieval was performed using citrate buffer pH 6 for 15 minutes.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab155768 has been referenced in 6 publications.
- Li S et al. Higher thymocyte selection-associated high mobility group box (TOX) expression predicts poor prognosis in patients with ovarian cancer. BMC Cancer 22:1216 (2022). PubMed: 36434543
- Yang M et al. TOX Acts as a Tumor Suppressor by Inhibiting mTOR Signaling in Colorectal Cancer. Front Immunol 12:647540 (2021). PubMed: 33897695
- Sekine T et al. TOX is expressed by exhausted and polyfunctional human effector memory CD8+ T cells. Sci Immunol 5:N/A (2020). PubMed: 32620560
- Khan O et al. TOX transcriptionally and epigenetically programs CD8+ T cell exhaustion. Nature 571:211-218 (2019). PubMed: 31207603
- Wei H et al. miRNA-135a regulates Hut78 cell proliferation via the GATA-3/TOX signaling pathway. Mol Med Rep 19:2361-2367 (2019). PubMed: 30747224
- Page N et al. Expression of the DNA-Binding Factor TOX Promotes the Encephalitogenic Potential of Microbe-Induced Autoreactive CD8+ T Cells. Immunity 48:937-950.e8 (2018). PubMed: 29768177