Overview

  • Product name
  • Description
    Rabbit polyclonal to TOX3
  • Tested applications
    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human TOX3 aa 221-250 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: O15405

  • Positive control
    • Mouse stomach tissue, Mouse lung tissue, and MDA-MB453 cell line lysates; Human brain tissue; WiDr cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab175129 is purified through a Protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 63 kDa.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

Images

  • Anti-TOX3 antibody (ab175129) at 1/100 dilution + Mouse stomach tissue lysate at 35 µg

    Predicted band size : 63 kDa
  • All lanes : Anti-TOX3 antibody (ab175129) at 1/100 dilution

    Lane 1 : MDA-MB453 cell line lysate
    Lane 2 : MDA-MB453 cell line lysate with blocking peptide

    Lysates/proteins at 12.5 µg per lane.


    Predicted band size : 63 kDa
  • All lanes : Anti-TOX3 antibody (ab175129) at 1/100 dilution

    Lane 1 : Mouse lung tissue lysate
    Lane 2 : Mouse lung tissue lysate with blocking peptide

    Lysates/proteins at 12.5 µg per lane.


    Predicted band size : 63 kDa
  • Immunohistochemical analysis of paraffin embedded Human brain tissue labeling TOX3 with ab175129 at 1/50 followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow Cytometrical analysis of WiDr cells labeling TOX3 with ab175129 at 1/10 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab175129 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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