Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesPeptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/128000.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 63 kDa.
    Preliminary experiments gave an approx 100kDa band in Human Brain (Cerebellum) lysates after 0.2µg/ml antibody staining. The 100kDa band was successfully blocked by incubation with the immunizing peptide.
    Please note that currently we cannot find an explanation in the literature for the size of the band we observe.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    References for Anti-TOX3 antibody (ab77432)

    ab77432 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry and for including the IHC and western blot images. It does seem the antibody is working as expected in western blotting. Until now we have not had any feedback regarding the use of ab77432 in IHC. I agree that you are ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"