Overview

  • Product nameAnti-TOX4 antibody
    See all TOX4 primary antibodies
  • Description
    Rabbit polyclonal to TOX4
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide: MEFPGGNDNY LTITGPSHPF LSGAETFHTP SLGDEEFEIP PISLDSDPSL, corresponding to a region within N terminal amino acids 1-50 of Human TOX4

  • Positive control
    • TOX4 transfected 293T lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab66651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 66 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/12500.

Target

Anti-TOX4 antibody images

  • All lanes : Anti-TOX4 antibody (ab66651) at 1.25 µg/ml

    Lane 1 : Molecular Weight Markers
    Lane 2 : Transfected 293T lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 66 kDa
    Observed band size : 74 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

References for Anti-TOX4 antibody (ab66651)

ab66651 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab66651.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"