Overview

  • Product nameAnti-TP53I11 antibody
  • Description
    Rabbit polyclonal to TP53I11
  • Specificityab65021 detects endogenous levels of total TP53I11 protein.
  • Tested applicationsSuitable for: ELISA, IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from an internal sequence of human TP53I11.

  • Positive control
    • Human brain tissue. HUVEC cell extracts.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab65021 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
ICC/IF Use a concentration of 1 - 5 µg/ml.

Target

  • RelevanceTP53I11 (Tumor protein p53 inducible protein 11), also referred to as PIG11 (p53-induced gene 11), is an early transcriptional target of tumor suppressor p53. It is up-regulated in the induction of apoptosis or cell growth inhibition by multiple chemopreventive agents. However, its biological role remains unclear.
  • Database links
  • Alternative names
    • p53 induced gene 11 protein antibody
    • p53 induced protein antibody
    • PIG 11 antibody
    • PIG11 antibody
    • Tumor protein p53 inducible protein 11 antibody
    see all

Anti-TP53I11 antibody images

  • ab65021, at 1/50 - 1/100 dilution, staining TP53I11 in human brain by immunohistochemistry using paraffin-embedded tissue, in the absence (left panel) or presence (right panel) of the immunising peptide.
  • All lanes : Anti-TP53I11 antibody (ab65021) at 1/500 dilution

    Lane 1 : HUVEC cell extract
    Lane 2 : HUVEC cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 19 kDa
    Observed band size : 19 kDa
  • ICC/IF image of ab65021 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65021, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-TP53I11 antibody (ab65021)

This product has been referenced in:
  • Noman MZ  et al. Hypoxia-Inducible miR-210 Regulates the Susceptibility of Tumor Cells to Lysis by Cytotoxic T Cells. Cancer Res 72:4629-4641 (2012). Read more (PubMed: 22962263) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"