Overview

  • Product name
    Anti-TP53TG5 antibody
  • Description
    Rabbit polyclonal to TP53TG5
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: RNRLRTVLKN LSLLKLLKSS NRRIQELHKL AKRCWHSLLS VPKILRISSG , corresponding to N terminal amino acids 36-85 of Human TP53TG5

  • Positive control
    • 721_B cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified by peptide affinity chromatography method.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    May play a significant role in p53/TP53-mediating signaling pathway.
  • Tissue specificity
    Highly expressed in heart, brain and small intestine. Less abundant in skeletal muscle, spleen, prostate, ovary and colon. A smaller transcript is expressed specifically in the testis.
  • Cellular localization
    Cytoplasm. Nucleus. Cell cycle dependent intracellular localization.
  • Information by UniProt
  • Database links
  • Alternative names
    • C20orf10 antibody
    • Chromosome 20 open reading frame 10 antibody
    • CLG01 antibody
    • dJ453C12.5 antibody
    • T53G5_HUMAN antibody
    • TP53 inducible gene 5 protein antibody
    • TP53 target 5 antibody
    • TP53 target gene 5 protein antibody
    • TP53-inducible gene 5 protein antibody
    • TP53-target gene 5 protein antibody
    • TP53TG5 antibody
    see all

Images

  • Anti-TP53TG5 antibody (ab81222) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 34 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

References

ab81222 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab81222.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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