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Full length native protein (purified) (Human).
Our Abpromise guarantee covers the use of ab14198 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution.|
|IHC-Fr||1/50 - 1/100.|
|IHC-P||1/50. Pretreatment with 0.05% Pronase E for 20 minutes is recommended.|
a14198 staining TPA Tissue Plasminogen Activator (A - blue) in Rat hippocampal/thalamic region tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde. Tissue was incubated in pre-block solution (10% normal hourse serum in antibody diluent (PBS + 0.1% Trition X-100)) for 1 hour. Samples were incubated with primary antibody (1/50 in PBS + pre-block solution) for 16 hours. B - trypsin (red), C - PAR2 (green), D - merge.
TPA Tissue Plasminogen Actvator and Trypsin co-localise.