Anti-Tissue Plasminogen Activator antibody (ab62763)
Key features and details
- Rabbit polyclonal to Tissue Plasminogen Activator
- Suitable for: ELISA, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
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Overview
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Product name
Anti-Tissue Plasminogen Activator antibody
See all Tissue Plasminogen Activator primary antibodies -
Description
Rabbit polyclonal to Tissue Plasminogen Activator -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant full length protein corresponding to Mouse TPA Tissue Plasminogen Activator.
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Positive control
- WB: Mouse TPA Tissue Plasminogen Activator (non-reduced).
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General notes
The concentration of this antibody is lot specific.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 0.82% Sodium phosphate, 0.0292% EDTA, 0.58% Sodium chloride -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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WB |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 62 kDa).
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Notes |
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ELISA
Use at an assay dependent concentration. |
WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 62 kDa). |
Target
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Function
Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration. -
Tissue specificity
Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk. -
Involvement in disease
Note=Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 fibronectin type-I domain.
Contains 2 kringle domains.
Contains 1 peptidase S1 domain. -
Domain
Both FN1 and one of the kringle domains are required for binding to fibrin.
Both FN1 and EGF-like domains are important for binding to LRP1.
The FN1 domain mediates binding to annexin A2.
The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site. -
Post-translational
modificationsThe single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.
Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.
N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.
Characterization of O-linked glycan was studied in Bowes melanoma cell line. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 18791 Mouse
- Entrez Gene: 25692 Rat
- SwissProt: P11214 Mouse
- SwissProt: P19637 Rat
- Unigene: 154660 Mouse
- Unigene: 107102 Rat
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Alternative names
- Alteplase antibody
- DKFZp686I03148 antibody
- Plasminogen activator tissue antibody
see all
Images
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All lanes : Anti-Tissue Plasminogen Activator antibody (ab62763) at 2 µg/ml
Lane 1 : Mouse TPA Tissue Plasminogen Activator (non-reduced) at 0.5 µg
Lane 2 : Mouse TPA Tissue Plasminogen Activator + 3 µg CPAI (non-reduced) at 0.5 µg
Lane 3 : Mouse TPA Tissue Plasminogen Activator (reduced) at 0.5 µg
Lane 4 : Mouse TPA Tissue Plasminogen Activator + 3ug CPAI (reduced) at 0.5 µg
Lane 5 : Marker
Secondary
All lanes : Anti Rabbit IgG ABC Kit
Predicted band size: 62 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 125 kDa, 38 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
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Datasheet download
References (3)
ab62763 has been referenced in 3 publications.
- Lenoir S et al. Post-synaptic Release of the Neuronal Tissue-Type Plasminogen Activator (tPA). Front Cell Neurosci 13:164 (2019). PubMed: 31105531
- Bonis A et al. Cardiovascular resistance to thrombosis in 13-lined ground squirrels. J Comp Physiol B 189:167-177 (2019). PubMed: 30317383
- Sagare AP et al. A lipoprotein receptor cluster IV mutant preferentially binds amyloid-ß and regulates its clearance from the mouse brain. J Biol Chem 288:15154-66 (2013). PubMed: 23580652