Anti-Tissue Plasminogen Activator antibody [H27B6] (ab28374)
Key features and details
- Mouse monoclonal [H27B6] to Tissue Plasminogen Activator
- Suitable for: WB, ELISA, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
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Overview
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Product name
Anti-Tissue Plasminogen Activator antibody [H27B6]
See all Tissue Plasminogen Activator primary antibodies -
Description
Mouse monoclonal [H27B6] to Tissue Plasminogen Activator -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISA, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant full length protein (Mouse)
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Epitope
protease domain (see Declerck et al) -
Positive control
- This antibody gave a positive result in IHC in the following FFPE tissue: Rat Normal Kidney.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 0.82% Sodium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Protein A Sepharose. Purity is = 98% assessed by visual inspection of a Coomassie® bluestained SDS-PAGE gel. -
Clonality
Monoclonal -
Clone number
H27B6 -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28374 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration. -
Tissue specificity
Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk. -
Involvement in disease
Note=Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 fibronectin type-I domain.
Contains 2 kringle domains.
Contains 1 peptidase S1 domain. -
Domain
Both FN1 and one of the kringle domains are required for binding to fibrin.
Both FN1 and EGF-like domains are important for binding to LRP1.
The FN1 domain mediates binding to annexin A2.
The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site. -
Post-translational
modificationsThe single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.
Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.
N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.
Characterization of O-linked glycan was studied in Bowes melanoma cell line. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 18791 Mouse
- Entrez Gene: 25692 Rat
- SwissProt: P11214 Mouse
- SwissProt: P19637 Rat
- Unigene: 154660 Mouse
- Unigene: 107102 Rat
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Alternative names
- Alteplase antibody
- DKFZp686I03148 antibody
- Plasminogen activator tissue antibody
see all
Images
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IHC image of TPA Tissue Plasminogen Activator staining in Rat Normal Kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28374, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Datasheets and documents
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Datasheet download
References (4)
ab28374 has been referenced in 4 publications.
- Cho MK et al. Zinc-triggered induction of tissue plasminogen activator and plasminogen in endothelial cells and pericytes. Exp Neurobiol 22:315-21 (2013). WB . PubMed: 24465147
- Hwang IY et al. Zinc-triggered induction of tissue plasminogen activator by brain-derived neurotrophic factor and metalloproteinases. J Neurochem 118:855-63 (2011). WB . PubMed: 21615740
- Cacquevel M et al. Ageing and amyloid-beta peptide deposition contribute to an impaired brain tissue plasminogen activator activity by different mechanisms. Neurobiol Dis 27:164-73 (2007). PubMed: 17566751
- Declerck PJ et al. Generation of monoclonal antibodies against autologous proteins in gene-inactivated mice. J Biol Chem 270:8397-400 (1995). PubMed: 7536737