Anti-TPA Tissue Plasminogen Activator antibody [T-1] (ab82249)


  • Product nameAnti-TPA Tissue Plasminogen Activator antibody [T-1]
    See all TPA Tissue Plasminogen Activator primary antibodies
  • Description
    Mouse monoclonal [T-1] to TPA Tissue Plasminogen Activator
  • Tested applicationsSuitable for: WB, IHC-Fr, ELISA, IP, Functional Studiesmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Native human TPA Tissue Plasminogen Activator

  • Epitopeab82249 binds to the A chain of TPA Tissue Plasminogen Activator.



Our Abpromise guarantee covers the use of ab82249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 63 kDa.
IHC-Fr Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration. Inhibits the enzymatic activity of TPA Tissue Plasminogen Activator. Can also be used for immunopurification


  • FunctionConverts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration.
  • Tissue specificitySynthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.
  • Involvement in diseaseNote=Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 fibronectin type-I domain.
    Contains 2 kringle domains.
    Contains 1 peptidase S1 domain.
  • DomainBoth FN1 and one of the kringle domains are required for binding to fibrin.
    Both FN1 and EGF-like domains are important for binding to LRP1.
    The FN1 domain mediates binding to annexin A2.
    The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site.
  • Post-translational
    The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.
    Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.
    N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.
    Characterization of O-linked glycan was studied in Bowes melanoma cell line.
  • Cellular localizationSecreted > extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alteplase antibody
    • DKFZp686I03148 antibody
    • Plasminogen activator tissue antibody
    • Plasminogen activator tissue type antibody
    • PLAT antibody
    • Reteplase antibody
    • t PA antibody
    • T Plasminogen Activator antibody
    • t-PA antibody
    • T-plasminogen activator antibody
    • Tissue plasminogen activator (t PA) antibody
    • Tissue type plasminogen activator antibody
    • Tissue-type plasminogen activator chain B antibody
    • tPA antibody
    • TPA_HUMAN antibody
    • TPA1 antibody
    see all

References for Anti-TPA Tissue Plasminogen Activator antibody [T-1] (ab82249)

This product has been referenced in:
  • Lund LR  et al. Plasminogen activator inhibitor type 1: cell-specific and differentiation-induced expression and regulation in human cell lines, as determined by enzyme-linked immunosorbent assay. Mol Cell Endocrinol 60:43-53 (1988). ELISA . Read more (PubMed: 3265112) »
  • Grøndahl-Hansen J  et al. Immunohistochemical localization of urokinase- and tissue-type plasminogen activators in psoriatic skin. J Invest Dermatol 88:28-32 (1987). IHC ; Human . Read more (PubMed: 3098860) »

See all 4 Publications for this product

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Thank you for contacting us. I would like to reassure you the quality of these antibodies may not have been affected by remaining at 4C during two weeks.

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Polyclonal antibodies are serum/plasma derived since they are taken from the blood of the animal. Monoclonal antibodies are generated by culturing the B cell clone that is producing the antibody in cell culture or ...

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/n not have a matching pair. However, I think we have one antibody which may interest you: (or use the following:
This antibody is derived ...

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