Anti-TPCN1 antibody (ab94731)

Overview

  • Product nameAnti-TPCN1 antibody
  • Description
    Rabbit polyclonal to TPCN1
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human, Zebrafish
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 72-121 (QEAAIYLQEG ENNDKFFTHP KDAKALAAYL FAHNHLFYLM ELATALLLLL) of Human TPCN1 (NP_060371).

  • Positive control
    • HT1080 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab94731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 94 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionNicotinic acid adenine dinucleotide phosphate (NAADP) receptor that may function as one of the major voltage-gated Ca(2+) channels (VDCC) across the lysosomal and endosomal membrane.
  • Tissue specificityHighest expression found in the heart and kidney, and lowest expression found in the spleen.
  • Sequence similaritiesBelongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. Two pore calcium channel subfamily.
  • DomainEach of the two internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
  • Cellular localizationLysosome membrane. Endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ20612 antibody
    • KIAA1169 antibody
    • TPC1 antibody
    • TPC1_HUMAN antibody
    • TPCN 1 antibody
    • TPCN1 antibody
    • Two pore calcium channel protein 1 antibody
    • Two pore channel 1 antibody
    • Two pore segment channel 1 antibody
    • Voltage dependent calcium channel protein TPC1 antibody
    • Voltage-dependent calcium channel protein TPC1 antibody
    see all

Anti-TPCN1 antibody images

  • Anti-TPCN1 antibody (ab94731) at 1 µg/ml (in 5% skim milk / PBS buffer) + HT1080 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 94 kDa
    Gel concentration: 6-18%
  • ab94731 staining TPCN1 in Zebrafish 1000-cell embryo by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 1% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/200 in BSA in PBS-T) for 18 hours at 4°C. An AlexaFluor® 488-conjugated Goat-anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

    See Abreview



  • Predicted band size : 94 kDa
    Western Blot using ab94731 at 1.0ug/ml. Sample type: Human HT1080 cell Lysate

References for Anti-TPCN1 antibody (ab94731)

ab94731 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Zebrafish Cell (1000-cell embryo)
Specification 1000-cell embryo
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Celeste Eno

Verified customer

Submitted Jun 19 2012

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"