Overview

  • Product nameAnti-TPD52 antibody
    See all TPD52 primary antibodies
  • Description
    Rabbit polyclonal to TPD52
  • Tested applicationsSuitable for: WB, IP, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow
  • Immunogen

    Synthetic peptide corresponding to the internal sequence of Human TPD52. This sequence is identical in Mouse, Rat, Human, Chicken and Bovine.

  • Positive control
    • MCF-7 cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.20
    Constituent: PBS
    Note: Contains protein stabilizer(0.05% PROCLIN300, 10MG/ML BSA)
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab125270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
IP Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.
IHC-P Use a concentration of 0.5 - 2 µg/ml.

Target

  • Tissue specificityIsoform 2 is expressed in colon, breast, prostate, pancreas and kidney tumor cell lines. Isoform 2 is expressed at high levels in kidney, prostate, brain, small intestine and pancreas, at moderate levels in placenta and colon, at low levels in lung, liver and heart, and at very low levels in spleen, thymus, peripheral mononuclear blood cells, testis and ovary.
  • Sequence similaritiesBelongs to the TPD52 family.
  • Developmental stageIsoform 2 is expressed at lower levels in fetal brain and kidney than in adult brain and kidney.
  • Information by UniProt
  • Database links
  • Alternative names
    • D52 antibody
    • hD52 antibody
    • Human 19.8 kDa protein mRNA, complete cds antibody
    • N8L antibody
    • PC 1 antibody
    • PC1 antibody
    • PC1/MRPS28 fusion protein antibody
    • PrLZ antibody
    • Prostate and colon associated protein antibody
    • Prostate leucine zipper antibody
    • Protein N8 antibody
    • TPD52 antibody
    • TPD52_HUMAN antibody
    • Tumor protein D52 antibody
    see all

Anti-TPD52 antibody images

  • Anti-TPD52 antibody (ab125270) at 1/500 dilution + MCF-7 cell lysate.

    Predicted band size : 24 kDa
    Observed band size : 24 kDa

References for Anti-TPD52 antibody (ab125270)

ab125270 has not yet been referenced specifically in any publications.

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry these products did not perform as stated on the datasheet and for the inconvenience th...

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Thank you for your reply.

I do understand your situation, butcanreplace only the 2 antibodies you had purchased. IP is untested for all the 3 antibodies you mentioned. If you like to try 2 of these antibodies in IP, you will be eligible for ...

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Thank you for your reply.

I am sorry to hear that the antibodies are not working as expected and that the tips and protocol sent did not help.

We do have 4 other TPD52 antibodies that are tested in WB and human, and which you could...

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Thank you for your patience.

For ab125270, I received the following information:

The protein should be separated under SDS-PAGE (denaturing conditions).The cell lysate derived from MCF-7was resolved onto 12% SDS-PAGE, transferred o...

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Thank you for your reply.

Since the antibodies did not give theexpected band size, you had requested the WB protocols for both antibodies as well as the transfer conditions.

For ab77459, we had sent you this information. For ab1252...

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Thank you for contacting us.

For both antibodies, I have contacted the lab for more WB protocol information, including information about the transfer.

Please let me know where you saw the band for the endogenous samples, or you cou...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"