The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000 - 1/20000. Detects a band of approximately 20 kDa (predicted molecular weight: 24 kDa).
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Isoform 2 is expressed in colon, breast, prostate, pancreas and kidney tumor cell lines. Isoform 2 is expressed at high levels in kidney, prostate, brain, small intestine and pancreas, at moderate levels in placenta and colon, at low levels in lung, liver and heart, and at very low levels in spleen, thymus, peripheral mononuclear blood cells, testis and ovary.
Belongs to the TPD52 family.
Isoform 2 is expressed at lower levels in fetal brain and kidney than in adult brain and kidney.