Overview

  • Product name
  • Description
    Rabbit polyclonal to TPD52
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Chicken, Cow, Pig
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-224 of Human TPD52 (UniProt: P55327).

  • Positive control
    • A549, H1299, HCT116 and MCF7 cell lysates; HBL435 xenograft
  • General notes
    Keep as a concentrated solution.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 79% PBS, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Detects a band of approximately 20 kDa (predicted molecular weight: 24 kDa).
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Tissue specificity
    Isoform 2 is expressed in colon, breast, prostate, pancreas and kidney tumor cell lines. Isoform 2 is expressed at high levels in kidney, prostate, brain, small intestine and pancreas, at moderate levels in placenta and colon, at low levels in lung, liver and heart, and at very low levels in spleen, thymus, peripheral mononuclear blood cells, testis and ovary.
  • Sequence similarities
    Belongs to the TPD52 family.
  • Developmental stage
    Isoform 2 is expressed at lower levels in fetal brain and kidney than in adult brain and kidney.
  • Information by UniProt
  • Database links
  • Alternative names
    • D52 antibody
    • hD52 antibody
    • Human 19.8 kDa protein mRNA, complete cds antibody
    • N8L antibody
    • PC 1 antibody
    • PC1 antibody
    • PC1/MRPS28 fusion protein antibody
    • PrLZ antibody
    • Prostate and colon associated protein antibody
    • Prostate leucine zipper antibody
    • Protein N8 antibody
    • TPD52 antibody
    • TPD52_HUMAN antibody
    • Tumor protein D52 antibody
    see all

Images

  • All lanes : Anti-TPD52 antibody (ab155296) at 1/10000 dilution

    Lane 1 : A549 whole cell lysate
    Lane 2 : H1299 whole cell lysate
    Lane 3 : HCT116 whole cell lysate
    Lane 4 : MCF7 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 24 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded HBL435 xenograft labeling TPD52 with ab155296 at 1/500 dilution.

References

ab155296 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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