The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesPeptide ELISA: Antibody detection limit dilution 1/8000.
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 24 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Tissue specificityIsoform 2 is expressed in colon, breast, prostate, pancreas and kidney tumor cell lines. Isoform 2 is expressed at high levels in kidney, prostate, brain, small intestine and pancreas, at moderate levels in placenta and colon, at low levels in lung, liver and heart, and at very low levels in spleen, thymus, peripheral mononuclear blood cells, testis and ovary.
Sequence similaritiesBelongs to the TPD52 family.
Developmental stageIsoform 2 is expressed at lower levels in fetal brain and kidney than in adult brain and kidney.