Overview

  • Product nameAnti-TPD52 antibody
    See all TPD52 primary antibodies
  • Description
    Mouse monoclonal to TPD52
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: SETLSQAGQK ASAAFSSVGS VITKKLEDVK NSPTFKSFEE KVENLKSKVG GTKPAGGDFG EVLNSAANAS ATTTEPLPEK TQESL, corresponding to amino acids 100-184 of human TPD52 (NP_005070) with a 26 kDa tag.

  • Positive control
    • TPD52 transfected 293T cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2b
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 24 kDa.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration. Detection limit for this product is ~1 ng/ml when used as a capture antibody.

Target

  • Tissue specificityIsoform 2 is expressed in colon, breast, prostate, pancreas and kidney tumor cell lines. Isoform 2 is expressed at high levels in kidney, prostate, brain, small intestine and pancreas, at moderate levels in placenta and colon, at low levels in lung, liver and heart, and at very low levels in spleen, thymus, peripheral mononuclear blood cells, testis and ovary.
  • Sequence similaritiesBelongs to the TPD52 family.
  • Developmental stageIsoform 2 is expressed at lower levels in fetal brain and kidney than in adult brain and kidney.
  • Information by UniProt
  • Database links
  • Alternative names
    • D52 antibody
    • hD52 antibody
    • Human 19.8 kDa protein mRNA, complete cds antibody
    • N8L antibody
    • PC 1 antibody
    • PC1 antibody
    • PC1/MRPS28 fusion protein antibody
    • PrLZ antibody
    • Prostate and colon associated protein antibody
    • Prostate leucine zipper antibody
    • Protein N8 antibody
    • TPD52 antibody
    • TPD52_HUMAN antibody
    • Tumor protein D52 antibody
    see all

Anti-TPD52 antibody images

  • Anti-TPD52 antibody (ab77459) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 24 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-TPD52 antibody (ab77459) at 5 µg/ml

    Lane 1 : TPD52 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 24 kDa
    Observed band size : 24 kDa
  • Detection limit for this product is ~1ng/ml as a capture antibody.
  • IHC image of ab77459 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77459, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-TPD52 antibody (ab77459)

ab77459 has not yet been referenced specifically in any publications.

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry these products did not perform as stated on the datasheet and for the inconvenience th...

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Thank you for your reply.

I do understand your situation, butcanreplace only the 2 antibodies you had purchased. IP is untested for all the 3 antibodies you mentioned. If you like to try 2 of these antibodies in IP, you will be eligible for ...

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Thank you for your reply.

I am sorry to hear that the antibodies are not working as expected and that the tips and protocol sent did not help.

We do have 4 other TPD52 antibodies that are tested in WB and human, and which you could...

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Thank you for your patience.

For ab125270, I received the following information:

The protein should be separated under SDS-PAGE (denaturing conditions).The cell lysate derived from MCF-7was resolved onto 12% SDS-PAGE, transferred o...

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Thank you for your reply.

Since the antibodies did not give theexpected band size, you had requested the WB protocols for both antibodies as well as the transfer conditions.

For ab77459, we had sent you this information. For ab1252...

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Thank you for contacting us.

For ab77459, the WB information is as follows:

The antibodyhas been tested in Western blot onTPD52 transfected 293T cell lysates and TPD52 (NP_005070, residues 100 -184) partial recombinant protein with...

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Thank you for contacting us.

For both antibodies, I have contacted the lab for more WB protocol information, including information about the transfer.

Please let me know where you saw the band for the endogenous samples, or you cou...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"