Recombinant
RabMAb

Anti-TPD52 antibody [EPR14220] (ab182578)

Overview

  • Product name
    Anti-TPD52 antibody [EPR14220]
    See all TPD52 primary antibodies
  • Description
    Rabbit monoclonal [EPR14220] to TPD52
  • Specificity
    Based on immunogen sequence analysis, ab182578 recognizes 4 isoforms with the predicted MWs of 24KDa, 20KDa, 26 KDa and 22KDa, respectively.
  • Tested applications
    Suitable for: WB, ICC/IF, IP, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TPD52 aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: P55327

  • Positive control
    • WB: SW480, HCT-116, Raji and THP1 cell lysates. IHC-P: Human prostatic hyperplasia and mouse kidney tissues. ICC/IF: MCF7 cells. Flow Cyt: Raji cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR14220
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab182578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 20-26 kDa (predicted molecular weight: 24 kDa).
ICC/IF 1/500.
IP 1/40 - 1/60.
IHC-P 1/50 - 1/100.
Flow Cyt 1/350.

Target

  • Tissue specificity
    Isoform 2 is expressed in colon, breast, prostate, pancreas and kidney tumor cell lines. Isoform 2 is expressed at high levels in kidney, prostate, brain, small intestine and pancreas, at moderate levels in placenta and colon, at low levels in lung, liver and heart, and at very low levels in spleen, thymus, peripheral mononuclear blood cells, testis and ovary.
  • Sequence similarities
    Belongs to the TPD52 family.
  • Developmental stage
    Isoform 2 is expressed at lower levels in fetal brain and kidney than in adult brain and kidney.
  • Information by UniProt
  • Database links
  • Alternative names
    • D52 antibody
    • hD52 antibody
    • Human 19.8 kDa protein mRNA, complete cds antibody
    • N8L antibody
    • PC 1 antibody
    • PC1 antibody
    • PC1/MRPS28 fusion protein antibody
    • PrLZ antibody
    • Prostate and colon associated protein antibody
    • Prostate leucine zipper antibody
    • Protein N8 antibody
    • TPD52 antibody
    • TPD52_HUMAN antibody
    • Tumor protein D52 antibody
    see all

Images

  • All lanes : Anti-TPD52 antibody [EPR14220] (ab182578) at 1/20000 dilution

    Lane 1 : HCT-116 cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : THP1 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 24 kDa
  • Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling TPD52 using ab182578 at 1/100 dilution. A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.

  • Immunofluorescent analysis of MCF7 cells labeling TPD52 using ab182578 at 1/500 dilution. A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Counterstain: DAPI.

  • Flow cytometry analysis of Raji (human Burkitt's lymphoma) cells labeling TPD52 (red) with ab182578 at a 1/350 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.

  • Western blot analysis of immunoprecipitation pellet from HCT-116 cell lysate (+) or a negative control (-) immunoprecipitated using ab182578 at 1/50 dilution.
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Anti-TPD52 antibody [EPR14220] (ab182578) at 1/10000 dilution + SW480 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 24 kDa
  • Immunohistochemical analysis of paraffin-embedded Human Prostatic hyperplasia tissue labeling TPD52 using ab182578 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

References

ab182578 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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