Anti-TPH2 antibody (ab111828)


  • Product nameAnti-TPH2 antibody
    See all TPH2 primary antibodies
  • Description
    Rabbit polyclonal to TPH2
  • Tested applicationsSuitable for: WB, IP, ICC, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human, Non human primates
  • Immunogen

    Synthetic peptide:


    , corresponding to N terminal amino acids 15-30 of Human TPH2.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium azide
    Constituent: 0.1% BSA
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab111828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IP 1/1000.
ICC 1/500.
IHC-FoFr Use at an assay dependent concentration.


  • Tissue specificityBrain specific.
  • PathwayAromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.
  • Involvement in diseaseGenetic variation in TPH2 may influence susceptibility to major depressive disorder (MDD) [MIM:608516].
    Defects in TPH2 are the cause of susceptibility to attention deficit-hyperactivity disorder type 7 (ADHD7) [MIM:613003]. ADHD is a neurobehavioral developmental disorder and is primarily characterized by the co-existence of attentional problems and hyperactivity, with each behavior occurring infrequently alone. Note=Naturally occurring variants of TPH2 with impaired enzyme activity could cause deficiency of serotonin production and result in an increased risk of developing behavioral disorders.
  • Sequence similaritiesBelongs to the biopterin-dependent aromatic amino acid hydroxylase family.
    Contains 1 ACT domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC138871 antibody
    • ADHD7 antibody
    • FLJ37295 antibody
    • MGC138872 antibody
    • Neuronal tryptophan hydroxylase antibody
    • NTPH antibody
    • TPH 2 antibody
    • Tph2 antibody
    • TPH2_HUMAN antibody
    • Tryptophan 5-hydroxylase 2 antibody
    • Tryptophan 5-monooxygenase 2 antibody
    • Tryptophan hydroxylase 2 antibody
    see all

Anti-TPH2 antibody images

  • Immunoprecipitation analysis of Raphe Nuclei isolated from Sprague-Dawley, precipitating ab111828 coupled to magnetic beads, which was introduced to whole cell extracts at room temperature for 15 mins. Immunoprecipitated proteins were assessed by Western blotting.


    Lane 1 (Positive Control): Non-immunoprecipitated brain lysates were probed with ab111828.
    Lane 2 (ab111828 Ms TPH2): Immunoprecipitated Proteins
    Lane 3 (Sheep TPH2 antibody): Immunoprecipitated Proteins
    Lane 4 (Negative Control => Anti-Tyrosine Hydroxylase antibody): Immunoprecipitated proteins

  • ab111828 staining TPH2 in Mouse dorsal raphe coronal brain slices tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.3% Triton X-100, blocked with 2% serum for 30 minutes at 22°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/800 in PBS + Triton X-100 + serum) at 22°C for 24 hours. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/500 in PBS + Goat serum) was used as the secondary antibody.

    See Abreview

References for Anti-TPH2 antibody (ab111828)

ab111828 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 22°C
Antigen retrieval step None
Sample Mouse Tissue sections (Dorsal Raphe coronal brain slices)
Specification Dorsal Raphe coronal brain slices
Permeabilization Yes - TritonX-100, 0.3%
Fixative Paraformaldehyde

Jason Teem

Verified customer

Submitted Nov 04 2013

DISCOUNT CODE: *** Expiration date: April 21st, 2012 I am very pleased to hear you would like to use ab111828 in IHC and send us an image of your results. This code will give you 1 free primary antibody before the expiration date. To redeem this offer,...

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Thank you very much for your interest in ab111828. Unfortunately, we would be unable to ship this antibody faster than 2-3 weeks, and we do not currently have an image of ab111828 in IHC. Therefore, I can offer a discount off a future purchase if you b...

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