Overview

  • Product nameAnti-TPH2 antibody
    See all TPH2 primary antibodies
  • Description
    Goat polyclonal to TPH2
  • Tested applicationsSuitable for: IHC-P, WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Horse, Hamster, Cow, Dog, Pig, Cynomolgus Monkey, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide:

    RGFSLDSAVPEEHQ-C

    corresponding to N terminal amino acids 16 - 29 of Human TPH2, wih a cysteine residue (NP_775489.2).

  • Positive control
    • HEK293 lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab121013 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/800 - 1/1500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa).
IHC-FoFr Use at an assay dependent concentration.

Target

  • Tissue specificityBrain specific.
  • PathwayAromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.
  • Involvement in diseaseGenetic variation in TPH2 may influence susceptibility to major depressive disorder (MDD) [MIM:608516].
    Defects in TPH2 are the cause of susceptibility to attention deficit-hyperactivity disorder type 7 (ADHD7) [MIM:613003]. ADHD is a neurobehavioral developmental disorder and is primarily characterized by the co-existence of attentional problems and hyperactivity, with each behavior occurring infrequently alone. Note=Naturally occurring variants of TPH2 with impaired enzyme activity could cause deficiency of serotonin production and result in an increased risk of developing behavioral disorders.
  • Sequence similaritiesBelongs to the biopterin-dependent aromatic amino acid hydroxylase family.
    Contains 1 ACT domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC138871 antibody
    • ADHD7 antibody
    • FLJ37295 antibody
    • MGC138872 antibody
    • Neuronal tryptophan hydroxylase antibody
    • NTPH antibody
    • TPH 2 antibody
    • Tph2 antibody
    • TPH2_HUMAN antibody
    • Tryptophan 5-hydroxylase 2 antibody
    • Tryptophan 5-monooxygenase 2 antibody
    • Tryptophan hydroxylase 2 antibody
    see all

Anti-TPH2 antibody images

  • IHC-P image of THP2 staining on mouse sections of Substantia Nigra. The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were them blocked using 1% BSA for 10 mins at 21°C. ab121013 was diluted 1:800and incubated with the sections for 2 hours at 21°C. The secondary antibody used was rabbit polyclonal to goat IgG conjugated to HRP.

  • IHC-P image of THP2 staining on rat sections of nigral-striatal track . The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were them blocked using 1% BSA for 10 mins at 21°C. ab121013 was diluted 1:1500 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was rabbit polyclonal to goat IgG conjugated to HRP.

  • Anti-TPH2 antibody (ab121013) at 0.5 µg/ml + HEK293 lysate in RIPA buffer at 35 µg

    Predicted band size : 56 kDa

References for Anti-TPH2 antibody (ab121013)

ab121013 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Brain)
Antigen retrieval step Heat mediated
Permeabilization No
Specification Brain
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Dec 03 2015

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 22°C
Antigen retrieval step None
Sample Rat Tissue sections (Brainstem)
Specification Brainstem
Permeabilization Yes - Triton-X 100
Fixative Paraformaldehyde
Username

Mr. Pieter Schipper

Verified customer

Submitted Oct 07 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Sep 26 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"