The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Preliminary experiments gave an approx 85kDa band in Mouse fetal Brain lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 55.9kDa according to NP_775567.2. The 85kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field.
Use a concentration of 0.5 - 2 µg/ml.
Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.
Involvement in disease
Genetic variation in TPH2 may influence susceptibility to major depressive disorder (MDD) [MIM:608516]. Defects in TPH2 are the cause of susceptibility to attention deficit-hyperactivity disorder type 7 (ADHD7) [MIM:613003]. ADHD is a neurobehavioral developmental disorder and is primarily characterized by the co-existence of attentional problems and hyperactivity, with each behavior occurring infrequently alone. Note=Naturally occurring variants of TPH2 with impaired enzyme activity could cause deficiency of serotonin production and result in an increased risk of developing behavioral disorders.
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. Contains 1 ACT domain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody (ab121020)Image courtesy Carl Hobbs (Kings College London, United Kingdom)
IHC-P image of THP2 staining on mouse sections of Substantia Nigra using ab121020 (1:800). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were them blocked using 1% BSA for 10 mins at 21°C. ab121020 was diluted 1:800and incubated with the sections for 2 hours at 21°C. The secondary antibody used was rabbit polyclonal to goat IgG conjugated to HRP.