Overview

  • Product nameAnti-Tpit antibody
    See all Tpit primary antibodies
  • Description
    Rabbit polyclonal to Tpit
  • Specificityab23475 reacts with T-box transcription factor (TBX19).
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Chimpanzee, Zebrafish
  • Immunogen

    A region within synthetic peptide: AMSELGTRKP SDGTVSHLLN VVESELQAGR EKGDPTEKQL QIILEDAPLW, corresponding to N terminal amino acids 2-51 of Human Tpit

  • Positive control
    • WB: Jurkat cell lysate IHC-P: human kidney and human liver sections.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab23475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 1.5 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceTpit (TBX19, T box 19) is a highly cell-restricted transcription factor belonging to the T-Box family. T box 19 is required for pro-opeiomelanocortin (POMC) gene expression and differentiation of corticotroph and melanotroph cells. Tpit gene mutations have been shown to be directly correlated to pituitary adrenocorticotropin (ACTH) deficiencies. Tpit functions as a transcriptional activator by recruiting the p160 family of coactivators.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • DJ747L4.1 antibody
    • T Box 19 antibody
    • T box factor, pituitary antibody
    • T box protein 19 antibody
    • T box transcription factor TBX19 antibody
    • TBS 19 antibody
    • TBS19 antibody
    • TBX19 antibody
    • TPIT antibody
    see all

Anti-Tpit antibody images

  • Anti-Tpit antibody (ab23475) at 2 µg/ml (Jurkat cell lysate)

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 48 kDa

References for Anti-Tpit antibody (ab23475)

ab23475 has not yet been referenced specifically in any publications.

Product Wall

Merci de votre intérêt pour ab23475.

Le marquage en IHC avec cet anticorps est en effet incorrect. Merci de nous avoir informés ce cette erreur.
J'ai demandé à ce que ces images soient retirées de...

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Thank you for contacting us for technical support for your customer, I'm sorry to hear he is experiencing problems with ab23475. This is the first complaint we have had with this antibody which gave us a single clear band around 50kDa in Jurkat lysate....

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"