Overview

  • Product name
  • Description
    Rabbit polyclonal to TPM4
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig, Zebrafish
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 54 and 226 of Human TPM4 (Uniprot: P67936)

  • Positive control
    • NT2D1, HeLa and Mouse brain lysate; 4T1 xenograft
  • General notes
    Keep as concentrated solution.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 28 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)

Target

  • Function
    Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Binds calcium.
  • Tissue specificity
    Detected in cardiac tissue and platelets, the form found in cardiac tissue is a higher molecular weight than the form found in platelets. Expressed at higher levels in the platelets of hypertensive patients with cardiac hypertrophy than in the platelets of hypertensive patients without cardiac hypertrophy (at protein level).
  • Sequence similarities
    Belongs to the tropomyosin family.
  • Domain
    The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • epididymis secretory protein Li 108 antibody
    • HEL-S-108 antibody
    • TM30p1 antibody
    • TMP 4 antibody
    • TPM4 antibody
    • TPM4_HUMAN antibody
    • Tropomyosin 4 antibody
    • Tropomyosin alpha 4 chain antibody
    • Tropomyosin alpha-4 chain antibody
    • Tropomyosin-4 antibody
    • wu:fb06e07 antibody
    • zgc:103436 antibody
    • zgc:63909 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded 4T1 xenograft labeling TPM4 with ab155293 at 1/100.
  • All lanes : Anti-TPM4 antibody (ab155293) at 1/1000 dilution

    Lane 1 : NT2D1 whole cell lysate
    Lane 2 : Hela whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 28 kDa
  • Anti-TPM4 antibody (ab155293) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size : 28 kDa

References

ab155293 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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