Overview

  • Product name
  • Description
    Rabbit polyclonal to TPM4
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human TPM4 (N terminal).
    Database link: NM_003290

  • Positive control
    • Skeletal muscle

Properties

Applications

Our Abpromise guarantee covers the use of ab93645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/200 - 1/500.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Binds calcium.
    • Tissue specificity
      Detected in cardiac tissue and platelets, the form found in cardiac tissue is a higher molecular weight than the form found in platelets. Expressed at higher levels in the platelets of hypertensive patients with cardiac hypertrophy than in the platelets of hypertensive patients without cardiac hypertrophy (at protein level).
    • Sequence similarities
      Belongs to the tropomyosin family.
    • Domain
      The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.
    • Cellular localization
      Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • epididymis secretory protein Li 108 antibody
      • HEL-S-108 antibody
      • TM30p1 antibody
      • TMP 4 antibody
      • TPM4 antibody
      • TPM4_HUMAN antibody
      • Tropomyosin 4 antibody
      • Tropomyosin alpha 4 chain antibody
      • Tropomyosin alpha-4 chain antibody
      • Tropomyosin-4 antibody
      • wu:fb06e07 antibody
      • zgc:103436 antibody
      • zgc:63909 antibody
      see all

    Images

    • ab93645, at 1/100 dilution, showing cytoplasmic staining of TPM4 in formalin-fixed, paraffin-embedded skeletal muscle by Immunohistochemistry.

    References

    ab93645 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Rh30)
    Loading amount
    20 µg
    Specification
    Rh30
    Gel Running Conditions
    Non-reduced Denaturing (NuPAGE 4-12% Bis-Tris Gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 14 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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