Overview

  • Product nameAnti-TPMT antibody [AT2E7]
    See all TPMT primary antibodies
  • Description
    Mouse monoclonal [AT2E7] to TPMT
  • Tested applicationsSuitable for: Flow Cyt, ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-245 of Human TPMT purified from E. coli (NP_000358).

  • Positive control
    • Hep3B cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab122984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 28 kDa.
ELISA Use at an assay dependent concentration.

Target

  • FunctionCatalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine.
  • Involvement in diseaseDefects in TPMT are the cause of thiopurine S-methyltransferase deficiency (TPMT deficiency) [MIM:610460]. TPMT is an enzyme involved in the normal metabolic inactivation of thiopurine drugs. These drugs are generally used as immunosupressants or cytotoxic drugs and are prescribed for a variety of clinical conditions including leukemia, autoimmune disease and organ transplantation. Patients with intermediate or no TPMT activity are at risk of toxicity after receiving standard doses of thiopurine drugs and it is shown that inter-individual differences in response to these drugs are largely determined by genetic variation at the TPMT locus.
  • Sequence similaritiesBelongs to the methyltransferase superfamily. TPMT family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • HGNC:12014 antibody
    • S adenosyl L methionine thiopurine S methyltransferase antibody
    • Thiopurine methyltransferase antibody
    • Thiopurine S methyltransferase antibody
    • Thiopurine S-methyltransferase antibody
    • TPMT antibody
    • TPMT_HUMAN antibody
    see all

Anti-TPMT antibody [AT2E7] images

  • Anti-TPMT antibody [AT2E7] (ab122984) at 1/1000 dilution + Hep3B lysate at 40 µg

    Secondary
    goat anti-mouse secondary antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 28 kDa


  • Predicted band size : 28 kDa

    The Recombinant Protein (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TPMT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

    Lane 1 : Recombinant Protein
    Lane 2 : HepG2 cell lysate

  • Immunocytochemistry/Immunofluorescence analysis of U87MG cells labelling TPMT with ab122984 at 1/100. An Alexa Fluor® 488-conjugated goat anti-mouse IgG was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

  • Flow Cytometry analysis of U87MG cells labelling TPMT with ab122984, staining at 2-5 µg/1x106 cells (red). A 488-conjugated goat anti-mouse IgG was used as the secondary antibody. Black - Isotype control, mouse IgG.

References for Anti-TPMT antibody [AT2E7] (ab122984)

ab122984 has not yet been referenced specifically in any publications.

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