Recombinant full length protein, corresponding to amino acids 1-245 of Human TPMT purified from E. coli (NP_000358).
Our Abpromise guarantee covers the use of ab122984 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/1000. Predicted molecular weight: 28 kDa.|
|ELISA||Use at an assay dependent concentration.|
The Recombinant Protein (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TPMT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1 : Recombinant Protein
Lane 2 : HepG2 cell lysate
Immunocytochemistry/Immunofluorescence analysis of U87MG cells labelling TPMT with ab122984 at 1/100. An Alexa Fluor® 488-conjugated goat anti-mouse IgG was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
Flow Cytometry analysis of U87MG cells labelling TPMT with ab122984, staining at 2-5 µg/1x106 cells (red). A 488-conjugated goat anti-mouse IgG was used as the secondary antibody. Black - Isotype control, mouse IgG.
ab122984 has not yet been referenced specifically in any publications.