Anti-TPOR/MPL antibody (ab232755)
Key features and details
- Rabbit polyclonal to TPOR/MPL
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-TPOR/MPL antibody
See all TPOR/MPL primary antibodies -
Description
Rabbit polyclonal to TPOR/MPL -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Mouse TPOR/MPL aa 50-300. Two N-terminal tags. (Expressed in E.coli).
Database link: Q08351 -
Positive control
- WB: HeLa whole cell lysate (ab150035); Recombinant mouse TPOR/MPL protein. IHC-P: Mouse lung, pancreas, placenta and spleen tissues.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232755 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.5 - 2 µg/ml.
|
|
IHC-P |
Use a concentration of 5 - 20 µg/ml.
|
Notes |
---|
WB
Use a concentration of 0.5 - 2 µg/ml. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
-
Function
Receptor for thrombopoietin. May represent a regulatory molecule specific for TPO-R-dependent immune responses. -
Tissue specificity
Expressed at a low level in a large number of cells of hematopoietic origin. Isoform 1 and isoform 2 are always found to be coexpressed. -
Involvement in disease
Defects in MPL are a cause of congenital amegakaryocytic thrombocytopenia (CAMT) [MIM:604498]. CAMT is a disease characterized by isolated thrombocytopenia and megakaryocytopenia with no physical anomalies. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 1 subfamily.
Contains 2 fibronectin type-III domains. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 4352 Human
- Entrez Gene: 17480 Mouse
- Omim: 159530 Human
- SwissProt: P40238 Human
- SwissProt: Q08351 Mouse
- Unigene: 82906 Human
- Unigene: 4864 Mouse
-
Alternative names
- C MPL antibody
- CD110 antibody
- mpl antibody
see all
Images
-
Anti-TPOR/MPL antibody (ab232755) at 2 µg/ml + Recombinant mouse TPOR/MPL protein
Secondary
HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution -
Anti-TPOR/MPL antibody (ab232755) at 2 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Secondary
HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution -
Formalin-fixed, paraffin-embedded mouse pancreas tissue stained for TPOR/MPL using ab232755 at 10 µg/ml in immunohistochemical analysis. DAB staining.
-
Formalin-fixed, paraffin-embedded mouse lung tissue stained for TPOR/MPL using ab232755 at 10 µg/ml in immunohistochemical analysis. DAB staining.
-
Formalin-fixed, paraffin-embedded mouse spleen tissue stained for TPOR/MPL using ab232755 at 10 µg/ml in immunohistochemical analysis. AEC staining.
-
Formalin-fixed, paraffin-embedded mouse placenta tissue stained for TPOR/MPL using ab232755 at 10 µg/ml in immunohistochemical analysis. AEC staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab232755 has not yet been referenced specifically in any publications.