Anti-TPP1 antibody (ab96498)
Key features and details
- Rabbit polyclonal to TPP1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-TPP1 antibody
See all TPP1 primary antibodies -
Description
Rabbit polyclonal to TPP1 -
Host species
Rabbit -
Specificity
This product detects Tripeptidyl-peptidase 1 (TPP1). It is unable to detect Adrenocortical dysplasia protein homolog which is also known as TPP1.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog -
Immunogen
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 224 and 562 of TPP1 (NP_000382)
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Positive control
- A431 whole cell lysate and H1299, HeLa, HepG2 lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96498 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 61 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 61 kDa. |
Target
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Function
Lysosomal serine protease with tripeptidyl-peptidase I activity. May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases. Requires substrates with an unsubstituted N-terminus. -
Tissue specificity
Detected in all tissues examined with highest levels in heart and placenta and relatively similar levels in other tissues. -
Involvement in disease
Defects in TPP1 are the cause of neuronal ceroid lipofuscinosis type 2 (CLN2) [MIM:204500]. A form of neuronal ceroid lipofuscinosis. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. The lipopigment pattern seen most often in CLN2 consists of curvilinear profiles. -
Sequence similarities
Belongs to the peptidase S53 family. -
Post-translational
modificationsActivated by autocatalytic proteolytical processing upon acidification. N-glycosylation is required for processing and activity. -
Cellular localization
Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 515575 Cow
- Entrez Gene: 485337 Dog
- Entrez Gene: 1200 Human
- Entrez Gene: 12751 Mouse
- Entrez Gene: 83534 Rat
- Omim: 607998 Human
- SwissProt: Q0V8B6 Cow
- SwissProt: Q9XSB8 Dog
see all -
Alternative names
- Cell growth inhibiting gene 1 protein antibody
- Cell growth-inhibiting gene 1 protein antibody
- Ceroid lipofuscinosis neuronal 2 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab96498 has been referenced in 3 publications.
- Huang K et al. Identification of three predictors of gastric cancer progression and prognosis. FEBS Open Bio 10:1891-1899 (2020). PubMed: 32735728
- Shlevkov E et al. A High-Content Screen Identifies TPP1 and Aurora B as Regulators of Axonal Mitochondrial Transport. Cell Rep 28:3224-3237.e5 (2019). PubMed: 31533043
- Chandra S et al. Aspirin Induces Lysosomal Biogenesis and Attenuates Amyloid Plaque Pathology in a Mouse Model of Alzheimer's Disease via PPARa. J Neurosci 38:6682-6699 (2018). PubMed: 29967008