Overview

  • Product name
    Anti-TPPP2 antibody
  • Description
    Rabbit polyclonal to TPPP2
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 8-150 of Human TPPP2.

  • Positive control
    • Human kidney tissue; TPPP2 transfected HEK293T lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    May bind tubulin but has no microtubule bundling activity.
  • Sequence similarities
    Belongs to the TPPP family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • C14orf8 antibody
    • Gm1790 antibody
    • Gm77 antibody
    • P18 antibody
    • p25beta antibody
    • Protein p25-beta antibody
    • RGD1566185 antibody
    • TPPP/p18 antibody
    • TPPP2 antibody
    • TPPP2_HUMAN antibody
    • Tubulin polymerization-promoting protein family member 2 antibody
    see all

Anti-TPPP2 antibody images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus and cytoplasm. Recommended concentration of ab121215 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121215 at 1/150 dilution staining TPPP2 in Paraffin-embedded Human Kidney tissue by Immunohistochemistry.
  • All lanes : Anti-TPPP2 antibody (ab121215) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : Over expression Lysate (Coexpressed with a C terminal myc DDK tag (~3.1 kDa) in mammalian HEK293T cells

References for Anti-TPPP2 antibody (ab121215)

ab121215 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab121215.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up