Anti-TPR antibody (ab70610)
Key features and details
- Rabbit polyclonal to TPR
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TPR antibody
See all TPR primary antibodies -
Description
Rabbit polyclonal to TPR -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Dog, Turkey, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla -
Immunogen
Synthetic peptide corresponding to a region between residue 1650 and 1700 of human TPR
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Positive control
- HeLa and 293T whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab70610 was affinity purified using an epitope specific to TPR immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70610 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 300 kDa (predicted molecular weight: 267 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 300 kDa (predicted molecular weight: 267 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Component of the cytoplasmic fibrils of the nuclear pore complex implicated in nuclear protein import. Its N-terminus is involved in activation of oncogenic kinases. Plays a role in the mitotic spindle checkpoint. -
Tissue specificity
Highest in testis, lung, thymus, spleen and brain, lower levels in heart, liver and kidney. -
Involvement in disease
Defects in TPR are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=Chromosomal aberrations involving TPR are found in thyroid papillary carcinomas. Intrachromosomal rearrangement that links the 5'-end of the TPR gene to the protein kinase domain of NTRK1 forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the carboxy terminus of the NTRK1 protein.
Note=Involved in tumorigenic rearrangements with the MET or RAF genes. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus > nuclear pore complex. Nucleus membrane. Chromosome > centromere > kinetochore. The assembly of the NPC is a stepwise process in which Trp-containing peripheral structures assemble after other components, including p62. Detected at kinetochores during prometaphase. - Information by UniProt
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Database links
- Entrez Gene: 7175 Human
- Entrez Gene: 108989 Mouse
- Entrez Gene: 304862 Rat
- Omim: 189940 Human
- SwissProt: P12270 Human
- SwissProt: F6ZDS4 Mouse
- SwissProt: F1MA98 Rat
- Unigene: 279640 Human
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Alternative names
- Nucleoprotein TPR antibody
- tpr antibody
- TPR_HUMAN antibody
- translocated promoter region (to activated MET oncogene) antibody
Images
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All lanes : Anti-TPR antibody (ab70610) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 267 kDa
Observed band size: 300 kDa why is the actual band size different from the predicted?
Additional bands at: 235 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
Detection of Human TPR by Western Blot of Immunprecipitate. ab70610 at 1µg/ml staining TPR in HeLa whole cell lysates immunoprecipitated using ab70610 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 1 second.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab70610 has been referenced in 3 publications.
- Lee ES et al. TPR is required for the efficient nuclear export of mRNAs and lncRNAs from short and intron-poor genes. Nucleic Acids Res 48:11645-11663 (2020). PubMed: 33091126
- López-Soop G et al. AKAP95 interacts with nucleoporin TPR in mitosis and is important for the spindle assembly checkpoint. Cell Cycle 16:947-956 (2017). PubMed: 28379780
- Myers KN et al. The bornavirus-derived human protein EBLN1 promotes efficient cell cycle transit, microtubule organisation and genome stability. Sci Rep 6:35548 (2016). WB ; Human . PubMed: 27739501