The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC/IF: 1/100 - 1/500. Permeabilization with Triton-X 100 is recommended.
IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionComponent of the cytoplasmic fibrils of the nuclear pore complex implicated in nuclear protein import. Its N-terminus is involved in activation of oncogenic kinases. Plays a role in the mitotic spindle checkpoint.
Tissue specificityHighest in testis, lung, thymus, spleen and brain, lower levels in heart, liver and kidney.
Involvement in diseaseDefects in TPR are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=Chromosomal aberrations involving TPR are found in thyroid papillary carcinomas. Intrachromosomal rearrangement that links the 5'-end of the TPR gene to the protein kinase domain of NTRK1 forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the carboxy terminus of the NTRK1 protein. Note=Involved in tumorigenic rearrangements with the MET or RAF genes.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus > nuclear pore complex. Nucleus membrane. Chromosome > centromere > kinetochore. The assembly of the NPC is a stepwise process in which Trp-containing peripheral structures assemble after other components, including p62. Detected at kinetochores during prometaphase.
ab84516 at a 1/250 dilution, staining in human TPR in human prostate carcinoma, by immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Detection: DAB staining using anti-Rabbit secondary antibody at a dilution of 1/100.
ab84516 at a 1:250 dilution, staining mouse TPR in mouse squamous cell carcinoma, by immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
Detection: DAB staining using anti-Rabbit secondary
antibody at a dilution of 1/100.
Immunocytochemistry - TPR antibody (ab84516)
ab84516 at a 1/250 dilution, staining human TPR in NBF-fixed asynchronous HeLa cells by immunocytochemistry.
Detection: anti-rabbit IgG-FITC conjugate used at a dilution of 1/100.
ab84516 at a 1/250 dilution, staining TPR in human breast carcinoma, by immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Detection: DAB staining using anti-Rabbit secondary antibody at a dilution of 1/100.
References for Anti-TPR antibody (ab84516)
This product has been referenced in:
Guo Y et al. Concentration-dependent lamin assembly and its roles in the localization of other nuclear proteins. Mol Biol Cell25:1287-97 (2014).
Read more (PubMed: 24523288) »
Umlauf D et al. The human TREX-2 complex is stably associated with the nuclear pore basket. J Cell Sci126:2656-67 (2013).
Read more (PubMed: 23591820) »